大鼠肝再生的肝脏炎症反应中骨髓瘤基因mRNA、微小RNA-540-3p、环状RNA_04996的表达变化与作用  

Expression change and role of myeloma cancer gene mRNA,microRNA-540-3p,circular RNA_04996 in the liver inflammatory reaction during the rat liver regeneration

作　　者：张春博 王改平[1,2] 王子慧 臧夏炎 薛奇杰 林凯琳 韩璐 王棋文[1,2] 徐存拴 ZHANG Chun-bo;WANG Gai-ping;WANG Zi-hui;ZANG Xia-yan;XUE Qi-jie;LIN Kai-lin;HAN Lu;WANG Qi-wen;XU Cun-shuan(College of Life Science,He’nan Normal University,He’nan Xinxiang 453007,China;State Key Laboratory Cultivation Base for Cell Differentiation Regulation,He’nan Xinxiang 453007,China)

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省-科技部共建细胞分化调控国家重点实验室培育基地,河南新乡453007

出　　处：《解剖学报》2023年第1期70-74,共5页Acta Anatomica Sinica

摘　　要：目的了解大鼠肝再生(LR)0 h和6 h时骨髓瘤基因(MYC)及其mRNA相互作用的微小RNA(miRNA,miR)和环状RNA(circRNA)的表达变化、相互作用和调节残肝炎症反应的途径与方式。方法按Higgins等方法制备大鼠2/3肝切除(PH)模型,用大规模定量检测技术测定大鼠肝再生中残肝的mRNA、miRNA和circRNA[合称内源竞争RNA(ceRNA)]表达变化,用Cytoscape 3.2软件构建ceRNA的相互作用网,用ceRNA综合分析等方法解析它们的表达相关性和作用相关性。结果PH后0 h和6 h时,MYC mRNA的比值为0.15±0.03和2.36±0.20,miR-540-3p为3.00±0.43和0.79±0.01,circRNA_04996为1.43±0.43和3.14±0.94。同时,PH后0 h时,MYC促进的纤溶酶原激活剂尿激酶受体(PLAUR)、肿瘤坏死因子(TNF)、白细胞介素1受体2型(IL1R2)等3个炎症反应相关基因表达下调,抑制的炎症反应相关基因利钠肽A(NPPA)、核受体亚家族O组B成员2(NROB2)和过氧化物酶体增殖物激活受体α(PPARA)表达上调。PH后6 h时,MYC促进的PLAUR、TNF、IL1R2等3个炎症反应相关基因表达上调,抑制的炎症反应相关基因NPPA、NROB2和PPARA表达下调。结论上述circRNA抑制的miRNA、miRNA抑制的MYC mRNA以及MYC调节的炎症反应相关基因的表达相关性和作用相关性有利于PH后0 h时残肝处于非炎症反应状态和PH后6 h时残肝处于炎症反应状态。Objective To explore the role pathway and pattern of the transcription factor myeloma cancer gene(MYC)and its mRNA interaction with microRNA(miRNA,miR)and ciccular RNA(circRNAs)at 0 hour and 6 hour in the rat liver regeneration.Methods The rat 2/3 hepatectomy(partial hepatectomy,PH)model was prepared as described by Higgins,the expression changes of mRNA,miRNA and circRNA[together named as competing endogenous RNAs(ceRNA)]of remnant liver were detected by the large-scale quantitative detection technology,the interaction network of ceRNA was constructed by Cytoscape 3.2 software,and their correlation in expression and role were analyzed by ceRNA comprehensive analysis.Results It was found that at 0 hour and 6 hours after PH,the ratio value of MYC mRNA showed 0.15±0.03 and 2.36±0.20,miR-540-3p displays 3.00±0.43 and 0.79±0.01,circRNA_04996 showed 1.43±0.43 and 3.14±0.94.At the same time,the four kinds of inflammatory reaction-related genes plasminogen activator urokinase receptor(PLAUR),tumor necrosis factor(TNF),interleukin 1 receptor type 2(IL1R2),ect,which were prometed in expression by MYC,were down-regulated at 0 hour after PH,but the inflammatory reaction-related genes natriuretic peptide A(NPPA),nuclear receptor subfamily O group B member 2(NROB2)and peroxisome proliferator activated receptor alpha(PPARA),which were inhibited in expression by MYC,were up-regulated at 0 hour after PH.On the other hand,the three kinds of inflammatory reaction-related genes PLAUR,TNF,IL1R2,ect,which are prometed in expression by MYC,were up-regulated at 6 hours after PH,but the inflammatory reaction-related genes NPPA,NROB2 and PPARA,which were inhibited in expression by MYC,were down-regulated at 6 hours after PH.Conclusion The correlation in expression and role of the miRNA,which are inhibited by circRNAs,MYC,its mRNA is inhibited by miRNAs,and the inflammatory reaction-related genes,which are regulated by MYC,and are helpful for the hepatocyte to be in noninflammatory reaction state at 0 hour after PH and to be in infl

关 键 词：肝再生 残肝炎症反应 骨髓瘤基因 生物高通量检测 内源竟争RNA综合分析 大鼠 

分 类 号：Q257[生物学—细胞生物学]