祛风宣痹方对哮喘大鼠气道炎症及MEK/ERK信号通路的影响  

Effects of Qufeng Xuanbi Prescription on Airway Inflammation and MEK/ERK Signaling Pathway in Asthma Rats

作　　者：王博寒 杨颖[1] 史锁芳[1] 刘丽[1] 汤玲玲 孙宪泓 WANG Bohan;YANG Ying;SHI Suofang;LIU Li;TANG Lingling;SUN Xianhong(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中国中医药信息杂志》2023年第3期74-78,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81774267、82004265);江苏省研究生科研创新计划项目(KYCX22_1940)。

摘　　要：目的观察祛风宣痹方对哮喘大鼠气道炎症及MEK/ERK信号通路的影响,探讨其改善哮喘的作用机制。方法将18只SD大鼠随机分为对照组、哮喘组和祛风宣痹方组,每组6只,采用卵清蛋白诱导哮喘模型,祛风宣痹方组予祛风宣痹方灌胃,对照组和哮喘组予等量生理盐水,连续14 d。HE染色观察大鼠肺组织病理变化,ELISA检测血清IgE、白细胞介素(IL)-6和肺泡灌洗液IL-6含量,Western blot检测肺组织MEK/ERK信号通路相关蛋白表达。将巨噬细胞RAW264.7分为对照组、脂多糖(LPS)组、祛风宣痹方组和U0126组,以LPS诱导细胞炎症反应,同时祛风宣痹方组和U0126组分别予祛风宣痹方或MEK通路抑制剂U0126干预,ELISA检测细胞培养液IL-6含量,Western blot检测细胞MEK/ERK信号通路相关蛋白表达。结果与对照组比较,哮喘组大鼠气道壁增厚,支气管周围炎症细胞增多,血清IgE、IL-6和肺泡灌洗液IL-6含量显著增加(P<0.01),肺组织p-MEK1/2、p-ERK1/2蛋白表达显著升高(P<0.05);与哮喘组比较,祛风宣痹方组大鼠气道炎症细胞浸润减少,血清IgE、IL-6和肺泡灌洗液IL-6含量显著减少(P<0.01),肺组织p-MEK1/2、p-ERK1/2蛋白表达显著降低(P<0.05)。细胞实验结果显示,与对照组比较,LPS组细胞培养液IL-6含量显著增加(P<0.01),细胞p-MEK1/2、p-ERK1/2蛋白表达显著升高(P<0.01);与LPS组比较,祛风宣痹方组细胞培养液IL-6含量显著减少(P<0.05),p-MEK1/2、p-ERK1/2蛋白表达显著降低(P<0.01)。结论祛风宣痹方能够缓解哮喘气道炎症,抑制MEK/ERK信号通路可能是其作用机制之一。Objective To study the effects of Qufeng Xuanbi Prescription on airway inflammation and MEK/ERK signaling pathway;To explore its mechanism of action in improving asthma.Methods 18 SD rats were randomly divided into control group,asthma group and Qufeng Xuanbi Prescription group,with 6 rats in each group.Rat asthma model was induced by ovalbumin.Qufeng Xuanbi Prescription group was intervened with Qufeng Xuanbi Prescription by gavage,and the control group and the asthma group were treated with the same amount of normal saline for 14 consecutive days.Pathological changes of rat lung tissue were observed by HE staining;the contents of IgE,IL-6 in serum and IL-6 in alveolar lavage fluid were detected by ELISA;Western blot was used to detect the expression of MEK/ERK signaling pathway-related proteins in lung tissue.Macrophage RAW264.7 cells were divided into control group,lipopolysaccharide(LPS)group,Qufeng Xuanbi Prescription group and U0126 group.LPS was used to induce cellular inflammatory response,and the Qufeng Xuanbi Prescription group and U0126 group were simultaneously intervened with Qufeng Xuanbi Prescription or the MEK pathway inhibitor U0126 respectively.ELISA was used to detect the content of IL-6 in cell culture medium,and Western blot was used to detect the expression of MEK/ERK signaling pathway-related proteins in cells.Results Compared with the control group,the airway wall of rats in the asthma group was thickened,the inflammatory cells around bronchus increased,the contents of IgE and IL-6 in serum and IL-6 in alveolar lavage fluid significantly increased(P<0.01),and the protein expressions of p-ERK1/2 and p-MEK1/2 in lung tissue were significantly increased(P<0.05).Compared with the asthma group,the inflammatory cell infiltration in Qufeng Xuanbi Prescription group was reduced,the contents of IgE and IL-6 in serum and IL-6 in alveolar lavage fluid significantly reduced(P<0.01),and the protein expressions of p-ERK1/2 and p-MEK1/2 in lung tissue were significantly decreased(P<0.05).Cell experiment

关 键 词：祛风宣痹方 气道炎症 支气管哮喘 MEK/ERK信号通路 大鼠 RAW264.7细胞 

分 类 号：R285.5[医药卫生—中药学]