两种荒漠蓝藻生长特征及其对培养水体微环境的影响  

Growth characteristics of two desert cyanobacteria and their influence on culture solution microenvironment

作　　者：王楠 赵燕翘 许文文 孙靖尧 李承义 赵洋 Wang Nan;Zhao Yanqiao;Xu Wenwen;Sun Jingyao;Li Chengyi;Zhao Yang(Shapotou Desert Research and Experiment Station,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000;University of Chinese Academy of Sciences,Beijing 100049)

机构地区：[1]中国科学院西北生态环境资源研究院沙坡头沙漠研究试验站,甘肃兰州730000 [2]中国科学院大学,北京100049

出　　处：《中国沙漠》2023年第1期66-74,共9页Journal of Desert Research

基　　金：国家自然科学基金项目(32171630);中国科学院“西部之光”重点项目。

摘　　要：人工生物土壤结皮是近年来新兴的绿色环保固沙材料,是目前沙区生态恢复研究的前沿和热点,用于人工生物土壤结皮培养的荒漠蓝藻等生物体的产量是该技术大规模应用的限制因素。为了解荒漠蓝藻的生长特征及其影响因子,以分离于腾格里沙漠蓝藻结皮中的优势荒漠蓝藻——具鞘微鞘藻(Microcolus vaginatus)和念珠藻(Nostoc carneum)为对象,探索了实验室条件下接种密度对荒漠蓝藻生长和培养水体pH及电导率的影响。结果显示:随着接种密度的增加,具鞘微鞘藻和念珠藻生长进入稳定期的时间提前。在生长初期,具鞘微鞘藻在0.35、0.95 mg·L^(-1)密度下的干重显著高于2.13、6.59、13.42 mg·L^(-1)密度下的干重,生长后期则相反。在生长前期和后期,念珠藻在0.04~0.32 mg·L^(-1)密度下的干重和总质量均显著高于0.86~2.59 mg·L^(-1)密度下;具鞘微鞘藻在6.59~13.42 mg·L^(-1)密度下的总质量显著高于0.35~2.13 mg·L^(-1)密度下;在相同接种密度条件时,具鞘微鞘藻的总质量显著高于念珠藻(P<0.05)。两种蓝藻的生长均提高了培养水体pH,但降低了电导率。具鞘微鞘藻和念珠藻的总质量与接种密度呈幂相关和二项式相关,培养水体pH与两种蓝藻的干重均呈线性相关。接种密度显著影响具鞘微鞘藻和念珠藻生长,两种蓝藻的大量繁殖是水体pH升高和电导率下降的主要因素。建议在进行大规模培养时两种荒漠蓝藻的接种密度范围分别为6.59~13.42 mg·L^(-1)和0.04~0.32 mg·L^(-1)。In recent years,the artificial biological soil crusts applied in land desertification has been developed to be a technology of new and green in the environment protection particular in fixing moving sand and the study of ecological restoration of desert areas.However,the yield of desert cyanobacteria and other organisms used for the cultivation of artificial biological soil crusts is a bottleneck that limits the large-scale application of this technology.Thereby,to explore the growth characteristics of desert cyanobacteria and their effect factors,seven predominated species isolating from Tengger Desert cyanobacteria crusts,including Microcolus vaginatus and Nostoc carneum,were selected as subjects.In the condition of laboratory test,the influence of inoculum density on its growth characteristics and the changes of pH and conductivity of water environment during growth are explored.Results showed that,with the increase of the inoculum density,the growth time to enter stationary phase of M.vaginatus and N.carneum was advanced.In the early stage of growth,the dry weight of M.vaginatus was significantly higher at densities of 0.35 and 0.95 mg·L^(-1) than at densities of 2.13,6.59 and 13.42 mg·L^(-1),and the opposite was true in the later stages of growth.In the early and late stages of growth,the dry weight of N.carneum in the density of 0.04-0.32 mg·L^(-1) was significantly higher than that of the density of 0.86-2.59 mg·L^(-1);the total mass of M.vaginatus at the density of 6.59-13.42 mg·L^(-1) was significantly higher than 0.35-2.13 mg·L^(-1),the total mass of Nostoc spp.was significantly higher at the density of 0.04-0.32 mg·L^(-1) than 0.86-2.59 mg·L^(-1),the total mass of M.vaginatus was significantly higher than Nostoc spp.under the same inoculum density.The growth of both M.vaginatus and N.carneum can increase the pH of water environment,but decrease the electrical conductivity significantly.The total mass of M.vaginatus and N.carneum showed a significant power and binomial relationship with inoculum

关 键 词：生物土壤结皮 荒漠蓝藻 具鞘微鞘藻(Microcolus vaginatus) 念珠藻(Nostoc carneum) 接种密度 

分 类 号：Q948.1[生物学—植物学]