基于UPLC-MS/MS研究炮制对白术芍药散8种成分药代动力学的影响  

Pharmacokinetics effect of 8 components of Baizhu Shaoyao San with processing method based on UPLC-MS/MS

作　　者：孙戡平 杨雪芬[1] 李静静 孟颖 金俊杰[3] Sun Kanping;Yang Xuefen;Li Jingjing;Meng Ying;Jin Junjie(Department of Pharmacy,Jinhu People's Hospital,Huaian Rehabilitation Hospital,Huaian 211600,China;Department of Pharmacy,Zhangjiagang Traditional Chinese Medicine Hospital,Nanjing University of Chinese Medicine,Zhangjiagang 215699,China;Research and Development Department,Nanjing Haichang Chinese Medicine Group Corporation,Nanjing University of Chinese Medicine,Nanjing 210061,China)

机构地区：[1]淮安市康复医院金湖县人民医院药学部,淮安211600 [2]南京中医药大学附属张家港中医医院药学部,张家港215699 [3]南京中医药大学附属南京海昌中药集团有限公司研发部,南京210061

出　　处：《国际中医中药杂志》2023年第1期60-66,共7页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81873004);江苏省科技成果转化专项资金项目(BA2018002);南京市科技计划项目(201812021);南京中医药大学自然科学基金项目(XZR2020100)。

摘　　要：目的探讨炮制对白术芍药散中8种成分在大鼠体内药代动力学的影响。方法将大鼠分为生品组与炒品组,分别灌胃生品及炒品组成方水煎液,定时进行眼底静脉采血并分离血浆,采用超高效液相色谱-串联质谱法(UPLC-MS/MS)检测2组大鼠血浆中白术芍药散8种特征性成分含量,并进行方法学考察。采用DAS软件对血药浓度及采血时间进行分析,获得不同组别的药-时曲线,计算并分析药动学参数。结果生品组与炒品组大鼠血清中8种成分的血液达峰浓度、达峰时间、药时曲线下面积、平均驻留时间均有不同程度上的差异。结论炮制对白术芍药散中8种成分在大鼠体内的药代动力学有所影响,可能会影响白术芍药散的临床疗效。Objective To explore the pharmacokinetics effect of 8 components of processed Baizhu Shaoyao San on rats.Methods The rats were divided into processing group and unprocessing group,administered with decoction of Baizhu Shaoyao San by gavage respectively.Then,blood was collected from fundus vein at certain time to obtain the plasma.Finally,the contents of 8 components in plasma were detected and compared by UPLC-MS/MS method,and the methodology of the experiment was tested.The drug concentration in blood and the collection time of blood were analyzed by DAS software,and the time curves of different groups were obtained,the pharmacokinetic parameters were calculated.Results The blood peak concentration,peak time,area under the drug time curve,and average residence time of 8 components in the serum of rats in the raw product group and the fried product group were different to varying degrees.Conclusion Processed Baizhu Shaoyao San could influence the behavior of the components measured in rats,which may affect the clinical therapeutic effect of Baizhu Shaoyao San.

关 键 词：药代动力学 白术芍药散 串联质谱法 炮制(中药) 中药化学成分 

分 类 号：R285.5[医药卫生—中药学]