嗜盐古菌启动子的研究及应用  

Research and application of haloarchaeal promoter

作　　者：徐旸 王锦鸿 徐桐 向华[1,2] 韩静 XU Yang;WANG Jinhong;XU Tong;XIANG Hua;HAN Jing(State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院微生物研究所、微生物资源前期开发国家重点实验,北京100101 [2]中国科学院大学生命科学学院,北京100049

出　　处：《微生物学报》2023年第2期523-539,共17页Acta Microbiologica Sinica

基　　金：国家重点研发计划(2020YFA0906800);国家自然科学基金(31970031,32170031);中国科学院中亚药物研发中心科研项目(CAM202202)。

摘　　要：嗜盐古菌是古菌域的一个重要代表类群,在遗传与代谢、进化与适应、前沿生物技术及合成生物学领域都显示了其重要的研究价值。嗜盐古菌启动子的认识和利用,可以为嗜盐古菌的基础和应用研究提供必要的条件。本文从古菌启动子的结构与功能出发,就启动子的研究方法、嗜盐古菌启动子的特征及嗜盐古菌启动子的应用3个方面综述了嗜盐古菌启动子的研究现状,并对嗜盐古菌启动子未来研究的重点和方向进行了展望。Halophilic archaea are an important group in the domain archaea.They are of great value in the fields of genetics and metabolism,evolution and adaptation,technology frontier and synthetic biology.Haloarchaeal promoter is indispensable for both fundamental and applied research on halophilic archaea.In this paper,we first described the structure and functions of archaeal promoter in transcription.Then,we summarized and discussed the research achievements in haloarchaeal promoter from the following three aspects:the methods for studying promoter,the constitutive and inducible haloarchaeal promoters reported so far,and their applications.The challenges and future research focusing on mining,designing,developing,and optimizing haloarchaeal promoter were also prospected.

关 键 词：嗜盐古菌 启动子 诱导型启动子 组成型启动子 

分 类 号：Q933[生物学—微生物学]