田菁根瘤菌YIC4027可溶性趋化受体Tlp1的功能鉴定  

Characterization of the soluble chemoreceptor Tlp1 in Sinorhizobium alkalisoli YIC4027

作　　者：黄炜娓 王艺璇 孙丽 董小燕 解志红 HUANG Weiwei;WANG Yixuan;SUN Li;DONG Xiaoyan;XIE Zhihong(College of Resources and Environment,Shandong Agricultural University,Tai’an 271018,Shandong,China;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,Shandong,China)

机构地区：[1]山东农业大学资源与环境学院,山东泰安271018 [2]中国科学院烟台海岸带研究所,山东烟台264003

出　　处：《微生物学报》2023年第2期775-785,共11页Acta Microbiologica Sinica

基　　金：国家重点研发计划(2019YFD1002702);国家自然科学基金(31870020);山东省重点研发计划(2021CXGC010804)。

摘　　要：【目的】初步探究田菁根瘤菌Sinorhizobium alkalisoli YIC4027中唯一含有PAS结构域可溶性趋化受体Tlp1的功能机理。【方法】本研究基于Red重组系统以及三亲接合技术进行缺失突变株的构建。对野生型和突变株的生长情况、趋化能力、趋氧性、细胞凝结、生物膜的形成、胞外多糖产量、在宿主根表的定殖及竞争性结瘤等表型进行了测定。【结果】与野生型相比,突变株的生长不受影响,趋化和趋氧能力降低,在宿主根表的定殖及竞争性结瘤能力降低,而细胞凝结能力、生物膜形成以及胞外多糖产生能力等均有所提高【。结论】本研究首次证实了S.alkalisoli YIC4027中可溶性趋化受体Tlp1影响细胞的趋化运动。[Objective]To decipher the functional mechanism of Tlp1 which is the only soluble chemoreceptor containing PAS domain in Sinorhizobium alkalisoli YIC4027.[Methods]We constructed the gene-deleted mutant strain based on the Red homologous recombination system and triparental conjugation.The growth rate,chemotaxis,aerotaxis,cell flocculation,biofilm formation,exopolysaccharide(EPS)production,colonization,and competitive nodulation of the wild type and the mutant strain were studied.[Results]Compared with the wild type,the mutant strain showed unaffected growth,weakened chemotaxis,aerotaxis,colonization,and competitive nodulation,and increased cell coagulation,biofilm formation,and EPS production.[Conclusion]This study confirmed that the soluble chemoreceptor Tlp1 in YIC4027 affected the chemotactic movement of the cells for the first time.

关 键 词：Sinorhizobium alkalisoli YIC4027 可溶性趋化受体 PAS结构域 趋化 

分 类 号：Q939.114[生物学—微生物学]