黄河滩地和稻田土中铁还原菌、不产氧光合细菌分布机制  

Distribution of ferric reducing bacteria and anoxygenic phototrophic bacteria in the Yellow River beach and paddy soil

作　　者：田莹莹 王强[2] 赵京 孙向辉 姬燕培 TIAN Yingying;WANG Qiang;ZHAO Jing;SUN Xianghui;JI Yanpei(College of Materials Science and Engineering,Henan Institute of Technology,Xinxiang 453003,Henan,China;State Key Laboratory of Crop Stress Adaptation and Improvement,Henan University,Kaifeng 475001,Henan,China)

机构地区：[1]河南工学院材料科学与工程学院,河南新乡453003 [2]河南大学作物逆境适应与改良国家重点实验室,河南开封475001

出　　处：《微生物学报》2023年第2期805-820,共16页Acta Microbiologica Sinica

基　　金：河南省高等学校重点科研项目(21A610003);河南工学院博士基金项目(9001/KQ1811);河南省科技攻关项目(202102210253,212102310079)。

摘　　要：【目的】探讨沿黄流域土壤中铁还原菌(ferric reducing bacteria,Fe RB)、不产氧光合细菌(anoxygenic phototrophic bacteria,An PB)的分布机制。【方法】以沿黄流域(原阳段)为研究对象,采集黄河滩地和稻田土样,利用16Sr RNA基因高通量测序和实时荧光定量分析技术,结合统计学分析,揭示Fe RB、An PB菌群结构、丰度和主要环境影响因子。【结果】二者中的优势Fe RB在科(属)水平为Hydrogenophilaceae(Thiobacillus)、Bacillaceae(Bacillus)、Clostridiaceae、Rhodobactereace(Rhodobacter)、Geobacteraceae(Geobacter),优势An PB为Rhodobactereace(Rhodobacter)、Chloroflexaceae(Chloronema)、Acetobacteraceae(Roseomonas)。An PB中Rhodobacteraceae与Fe RB中Bacillaceae、Clostridiaceae的相对丰度负相关;An PB中Sphingomonadaceae与Hydrogenophilaceae、Clostridiaceae的相对丰度亦负相关。土壤硝酸盐氮(NO_(3)^(-)-N)与Rhodobactereace相对丰度负相关,与Geobacteraceae相对丰度正相关。二价铁(Fe2+)对Fe RB、An PB菌群组成的差异分别可解释13.5%、41.8%,p H对Fe RB、An PB菌群组成的差异分别可解释65.7%、42.8%。黄河滩地总细菌(total bacteria,BAC)、地(热)杆菌[Geo(thermo)bacter,GEO]、光合紫细菌(phototrophicpurplebacteria,PPB)的拷贝数分别为2.52(±3.43)×109、5.21(±7.58)×10^(7)、2.9(±3.70)×10^(7)copies/g干土。稻田土中BAC、GEO、PPB拷贝数依次为3.82(±1.29)×10^(10)、3.05(±2.44)×10^(8)、4.31(±0.90)×10^(8)copies/g干土。0-1 cm土层中PPB拷贝数显著高于1-2 cm、2-3cm土层。Fe2+对BAC、GEO、PPB数量分布变异的解释度为81.5%。【结论】土壤类型不同,潜在Fe RB、An PB物种组成不同,GEO、PPB丰度也不同。Fe2+对Fe RB、An PB分布起关键驱动作用。[Objective]To explore the distribution of ferric reducing bacteria(FeRB)and anoxygenic phototrophic bacteria(AnPB)in the soil along the Yellow River.[Methods]Soil samples were collected from the beach and paddy fields at the Yuanyang section of the Yellow River.High-throughput sequencing of 16S rRNA gene and quantitative real-time PCR were combined with statistical analysis to reveal the structure and abundance of FeRB and AnPB and the main environmental factors affecting the bacteria.[Results]The dominant FeRB families(genera)were Hydrogenophilaceae(Thiobacillus),Bacillaceae(Bacillus),Clostridiaceae,Rhodobactereace(Rhodobacter)and Geobacteraceae(Geobacter).The dominant AnPB families(genera)were Rhodobactereace(Rhodobacter),Chloroflexaceae(Chloronema)and Acetobacteraceae(Roseomonas).The relative abundance of Rhodobacteraceae(AnPB)was negatively correlated with that of Bacillaceae and Clostridiaceae(FeRB).The relative abundance of Sphingomonadaceae(AnPB)was negatively correlated with that of Hydrogenophilaceae and Clostridiaceae(FeRB).Soil nitrate nitrogen(NO_(3)^(-)-N)was negatively correlated with the relative abundance of Rhodobacteraceae but positively correlated with that of Geobacteraceae.Ferrous ions(Fe2+)explained 13.5%and 41.8%of the community variations of FeRB and AnPB,respectively;pH explained 65.7%and 42.8%,respectively.The number of total bacteria(BAC),Geo(thermo)bacter(GEO)and phototrophic purple bacteria(PPB)in the Yellow River beach was 2.52(±3.43)×10~9,5.21(±7.58)×10^(7) and 2.9(±3.70)×10^(7) copies/g dry soil,respectively,and that in the paddy soil was 3.82(±1.29)×10^(10),3.05(±2.44)×10~8 and 4.31(±0.90)×10~8 copies/g dry soil,respectively.Moreover,the PPB in the upmost soil layer(0-1 cm)were significantly more than those in the 1-2 cm and 2-3 cm soil layers.Fe2+explained 81.5%variations in the absolute abundance of BAC,GEO and PPB.[Conclusion]The potential community of FeRB and AnPB and the abundance of GEO and PPB varied between different soil types.Overall,Fe2+played a key role in

关 键 词：铁还原菌 不产氧光合细菌 稻田土 黄河滩地 

分 类 号：S154.3[农业科学—土壤学]