蜂毒肽浓度和磷脂组分对巨囊泡泄露的影响  

Effects of melittin concentration and phospholipid composition on giant unilamellar vesicles

作　　者：姜建功 盛洁 石铭芸 蒋中英 JIANG Jian-Gong;SHENG Jie;SHI Ming-Yun;JIANG Zhong-Ying(Key Laboratory of Micro-Nano Electronic Sensing Technology and Bionic Devices,Yili Normal University,Yining 835000,China;College of Electronics and Engineering,Yili Normal University,Yining 835000,China;College of Network Security and Information Technology,Yili Normal University,Yining 835000,China)

机构地区：[1]伊犁师范大学微纳电传感技术与仿生器械重点实验室,伊宁835000 [2]伊犁师范大学电子与工程学院,伊宁835000 [3]伊犁师范大学网络安全与信息技术学院,伊宁835000

出　　处：《原子与分子物理学报》2023年第6期29-34,共6页Journal of Atomic and Molecular Physics

基　　金：新疆维吾尔自治区自然科学基金(2020D01C266);伊犁师范大学博士科研启动基金(2020YSBS006);国家自然科学基金(22163011);新疆维吾尔自治区研究生科研创新项目(XJ2020G302);伊犁师范大学研究生科研创新项目(YS2022G001);新疆维吾尔自治区研究生科研创新项目(XJ2022G230)。

摘　　要：蜂毒肽作为一种广谱抗菌肽已经得到广泛认知,用蜂毒肽构建载药体系攻击癌细胞研究正在兴起.基于仿生物膜模型探索其破坏机理,可以避免潜在活性细胞过程的影响.在此,我们选用细胞尺寸的单层巨囊泡膜模型,可在光学显微镜下直接观察和操作,获得仿正常细胞膜和仿癌细胞膜在不同蜂毒肽浓度刺激下的响应.研究得出,低浓度蜂毒肽诱导囊泡泄露实验表明中性磷脂囊泡以孔模式为主泄露,负电性磷脂囊泡以爆裂模式为主泄露;高浓度蜂毒肽诱导囊泡泄露实验表明负电性磷脂相较于中性磷脂可延迟蜂毒肽作用效果;蜂毒肽色氨酸残基荧光光谱表明囊泡膜表面蜂毒肽吸附量以及泄露模式依赖于磷脂组分.此外,推断了蜂毒肽对不同组分磷脂膜的破坏作用模型.研究为蜂毒肽在肿瘤细胞的作用机制及其衍生物的优化设计提供参考.Melittin has been widely recognized as a broad-spectrum antibacterial peptide.The research of using melittin to construct drug delivery system to attack cancer cells is rising.Exploring its destruction mechanism based on biomimetic membrane model can avoid the influence of potentially active cell processes.Here,we choose giant unilamellar vesicles membrane model with cell size,which can be observed and operated directly under the optical microscope to obtain the responses of mimicking normal cell membrane and cancer cell membrane stimulated by different melittin concentrations.The results showed that the leakage of neutral phospholipid vesicles was mainly in pore mode,and the leakage of negative phospholipid vesicles was mainly in burst mode.The experiment of vesicle leakage induced by high concentration melittin showed that negative phospholipids could delay the effect of melittin compared with neutral phospholipids.The fluorescence spectra of melittin tryptophan residues showed that the adsorption amount and leakage mode of melittin on the surface of vesicle membrane depended on the phospholipid component.In addition,the destruction models of melittin on phospholipid membrane of different components were deduced.This study provides a reference for the action mechanism of melittin in tumor cells and the optimal design of its derivatives.

关 键 词：蜂毒肽 中性磷脂 负电性磷脂 泄露 

分 类 号：O485[理学—固体物理]