基于网络药理学探讨清心滋肾汤治疗围绝经期综合征的作用机制  

Study on the mechanism of Qingxin Zishen decoction in the treatment of perimenopausal syndrome based on network pharmacology

作　　者：郭倩 张秦 管群 GUO Qian;ZHANG Qin;GUAN Qun(Gynecology Department,Jiangsu Province Hospital of Chinese Medicine,The Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210029,China)

机构地区：[1]江苏省中医院、南京中医药大学附属医院妇科,江苏南京210029

出　　处：《安徽医药》2023年第3期620-625,I0004,I0005,共8页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 探讨清心滋肾汤治疗围绝经期综合征(MPS)的作用机制。方法 该研究起止时间为2021年1—6月。运用TCMSP数据库检索清心滋肾汤中各药物的活性成分;通过GeneCards等数据库获取围绝经期综合征疾病靶点,两者取交集后得到清心滋肾汤治疗MPS的预测靶点,接着构建“药物-成分-靶点-疾病”网络以及蛋白互作网络(PPI)和精简核心网络;运用R软件对清心滋肾汤治疗MPS进行基因本体论(GO)富集分析和京都基因和基因组数据库(KEGG)通路富集分析。结果 筛选得到69个主要活性成分,包括木犀草素(Luteolin)、槲皮素(Quercetin)、豆甾醇(Stigmasterol)、谷固醇(Beta-sitosterol)、山柰酚(Kaempferol)、四氢鸭脚木碱(Tetrahydroalstonine)等;54个靶点基因,核心靶点基因包括白细胞介素(IL)-6、IL-1β、雌激素受体-1(ESR1)、人纤溶酶原激活物抑制剂1(SERPINE1)、过氧化物酶体增生激活受体γ(PPARG)、补体调节蛋白(CRP)、细胞周期蛋白D1(CCND1)、孕激素受体(PGR)、血红素加氧酶1(HMOX1)、血管细胞黏附分子-1(VCAM-1)、细胞色素P450芳香化酶(CYP19A1)、胰岛素样生长因子结合蛋白3(IGFBP3)等;富集到GO生物过程(BP)1 180条、细胞定位(CC)39条、分子功能(MF)61条;KEGG通路162条,包括糖尿病并发症中AGE-RAGE信号通路、脂质和动脉粥样硬化信号通路、PI3K-Akt信号通路、流体剪切应力和动脉粥样硬化通路等。结论 清心滋肾汤通过木樨草素、槲皮素、豆甾醇、谷固醇、山柰酚、四氢鸭脚木碱等有效成分作用于IL-6、IL-1β、ESR1、SERPINE1、PPARG、CRP、CCND1、PGR、HMOX1、VCAM-1、CYP19A1、IGFBP3等关键靶点通过调节糖脂代谢、抗氧化、改善雌激素代谢等作用机制达到治疗围绝经期综合征的作用。Objective To explore the mechanism of Qingxin Zishen decoction in the treatment of perimenopausal syndrome(MPS).Methods The study started from January to June 2021. By searching the TCMSP database, the active ingredients of every drug in Qingxin Zishen decoction were acquired. The perimenopausal syndrome disease targets were collected from databases like GeneCards and so on;the predictive target of Qingxin Zishen decoction for treating MPS was obtained after the intersection of the two. A "drug-component-target-disease" network and a protein interaction network(PPI) were constructed, and then the core network was streamlined. Go enrichment analysis and KEGG pathway enrichment analysis of Qingxin Zishen decoction in the treatment of MPS were carried out by R software.Results 69 main active ingredients were screened, including Luteolin, Quercetin, Stigmasterol, Beta-sitosterol, Kaempferol, Tetrahydroalstonine etc.;54 gene targets were screened, the core target genes included IL-6, IL-1β, ESR1, SERPINE1, PPARG,CRP, CCND1, PGR, HMOX1, VCAM-1, CYP19A1, IGFBP3, etc.;GO enrichment items were obtained, including the 1 180 Biological Process(BP), 39 Cellular Component(CC), 61 Molecular Function(MF). There were 162 KEGG pathways, including AGE-RAGE signaling pathway in diabetic complications, lipid and atherosclerosis signaling pathway, PI3K-Akt signaling pathway, fluid shear stress and atherosclerosis pathway.Conclusion Qingxin Zishen decoction acts on key targets like IL-6, IL-1β, ESR1, SERPINE1, PPARG,CRP, CCND1, PGR, HMOX1, VCAM-1, CYP19A1 and IGFBP3 by regulating glycolipid metabolism, antioxidation, and improving the mechanism of estrogen metabolism to treat perimenopausal syndrome.

关 键 词：围绝经期综合征 木樨草素 槲皮素 清心滋肾汤 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]