土木香内酯抑制骨肉瘤细胞生长作用研究  

Inhibition Effects of Alantolactone on the Proliferation of Human Osteosarcoma cells

作　　者：王晓冬 李鑫 童晓琼 于春雷 袁斌[2] WANG Xiao-dong;LI Xin;TONG Xiao-qiong;YU Chun-lei;YUAN Bin(Nanchong Central Hospital,The Second Clinical Medical College,North Sichuan Medical College,Nanchong 637000,China;School of Pharmacy,North Sichuan Medical College,Institute of Materia Medica,Nanchong 637000,China;Nanchong City Key Laboratory of Personalized Drug Treatment,Nanchong 637000,China;School of Nursing Affiliated Hospital,North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]南充市中心医院,川北医学院第二临床医学院,四川南充637000 [2]川北医学院药学院药物研究所,四川南充637000 [3]个体化药物治疗南充市重点实验室,四川南充637000 [4]川北医学院附属医院护士学校,四川南充637000

出　　处：《中国药学杂志》2023年第2期151-159,共9页Chinese Pharmaceutical Journal

基　　金：公立医院高质量发展试点中央补助2022(个体化药物治疗实验室建设)项目资助(ZX-2208-2)。

摘　　要：目的研究土木香内酯(alantolactone,ALT)对骨肉瘤细胞株143B和saos-2的恶性增殖抑制作用。方法采用CCK-8和EdU法检测ALT对143B和saos-2细胞增殖的影响;细胞划痕法、Transwell侵袭法分别检测ALT对143B和saos-2细胞的迁移、侵袭能力的影响,流式细胞术检测ALT对143B和saos-2细胞周期和凋亡的影响;qPCR和WB法分别检测ALT对143B和saos-2细胞中LAT1、SKA2、BUB1B、BCL2、STAT3mRNA和蛋白表达量的影响。结果ALT能够抑制143B、saos-2细胞增殖、迁移和侵袭,将细胞阻滞于G1期,并促进细胞凋亡(P<0.05或P<0.01)。与对照组相比,ALT作用后,143B、saos-2细胞中LAT1 mRNA和蛋白、SKA2与BUL1B mRNA和蛋白水平显著下调,BCL2与STAT3 mRNA和蛋白水平显著下调(P<0.05或P<0.01)。结论ALT可抑制骨肉瘤143B和saos-2细胞的增殖迁移与侵袭,并将细胞阻滞于G1期,诱导其凋亡,其机制可能与抑制氨基酸转运(LAT1)、抑制细胞周期调控(SKA2、BULB1)、促进凋亡(STAT3、BCL2)等多环节有关。OBJECTIVE To investigate the inhibitory effect of alantolactone(ALT)on the proliferation of 143B and saos-2 cells of osteosarcoma cells.METHODS The proliferation and vitality effect of ALT on 143B and saos-2 cells were evaluated by CCK-8 and EdU assay.The invasion and migration effect of ALT on 143B and saos-2 cells were detected by cell invasion and cell scratch method.The effects of ALT on the cell cycle and apoptosis of 143B and saos-2 cells were detected by flow cytometry.The effects of ALT on LAT1,SKA2,BUB1B,BCL2 and STAT3 mRNA and protein levels in 143B and saos-2 cells were measured by qPCR and WB.RESULTS ALT could significantly inhibit the proliferation,migration and invasion of 143B and saos-2 cells.ALT could block cells at G1 phase,and induce the apoptosis of 143B and saos-2 cells(P<0.05 or P<0.01).Compared with the control group,the expressions of LAT1mRNA and protein,SKA2 and BUB1BmRNA and protein,BCL2 and STAT3mRNA and protein in 143B and saos-2 cells were all decreased by ALT,and the difference was statistically significant(P<0.05 or P<0.01).CONCLUSION ALT can inhibit the proliferation,migration and invasion of osteosarcoma 143B and saos-2 cells,and induce apoptosis and arrest the cell cycle of 143B and saos-2 in G1 phase,and its mechanism may be related to multiple links such as inhibiting amino-acid transport(LAT1),regulating cell cycle(SKA2,BUB1B),and promoting cell apoptosis(STAT3,BCL2).

关 键 词：骨肉瘤 土木香内酯 迁移 侵袭 增殖 凋亡 

分 类 号：R965[医药卫生—药理学] R285.5[医药卫生—药学]