独活化学成分及抗骨质疏松活性评价  

Studies on the chemical constituents from the roots of Angelicae pubescentis and their inhibition of osteoclastogenesis

作　　者：曹刘丹 胡晨 顾琼[2] 何琳[3] 滕希峰 田素英[3] 李钟 CAO Liu-dan;HU Chen;GU Qiong;HE Lin;TENG Xi-feng;TIAN Su-ying;LI Zhong(School of Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhou 510006,China;Guangdong Provincial Cosmetics Engineering and Technology Research Center,Guangdong Pharmaceutical University,Zhongshan 528458,China;State Administration of Traditional Chinese Medicine Key Laboratory for Production and Development of lingnan Medicinal Materials,Guangzhou 510006,China)

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]中山大学药学院,广东广州510006 [3]广东药科大学广东省化妆品工程技术研究中心,广东中山528458 [4]国家中医药管理局岭南药材生产与开发重点研究室,广东广州510006

出　　处：《时珍国医国药》2022年第12期2918-2923,共6页Lishizhen Medicine and Materia Medica Research

基　　金：广东省自然科学基金(2019A1515012138)。

摘　　要：目的研究独活(Angelicae pubescentis)根的化学成分及其抗骨质疏松活性。方法采用硅胶、RP-18、Sephadex LH-20等层析柱及半制备高效液相色谱对独活根部分95%乙醇提取物进行分离纯化,通过核磁共振波谱、质谱以及与文献数据比较,进行结构鉴定。结果从独活中分离得到20个化合物,分别鉴定为蛇床子素(1),isomeranzine(2),欧芹酚(3),meranzin(4),补骨脂素(5),香柑内酯(6),氧化前胡素水合物(7),(+)-visamminol(8),二氢欧山芹醇乙酸酯(9),二氢欧山芹素(10),二氢欧山芹醇(11),hydroxylomatin(12),当归醇A(13),angelmarin(14),没药当归烯酮(15),decursidate(16),(11S,16S,9Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol,1-acetate(17),(11S,16S,9Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol(18),9,12-十八烷二烯酸甲脂(19)和ligustiphenol(20)。结论化合物4,8,12,16,17,18和20为首次从该植物中分离得到。对20个化合物进行抗骨质疏松活性筛选,发现5个化合物(5,6,14,15和18)具有显著抑制RANKL诱导破骨细胞分化的活性。Objective To investigate the chemical constituents of the roots of Angelicae pubescentis and their inhibitory effects on osteoclastic cell formation activity.Methods These compounds were isolated and purified by silica gel,RP-18 column chromatography,Sephadex LH-20 column chromatography and semi-preparative HPLC.Their structures were identified by NMR,MS and comparison with literature data.Results The structure of twenty compounds were identified as osthol(1),isomeranzine(2),osthenol(3),meranzin(4),psoralen(5),bergapten(6),oxypeucedanin hydrate(7),(+)-visamminol(8),columbianetin acetate(9),columbianadin(10),columbianetin(11),hydroxylomatin(12),angelol A(13),angelmarin(14),bisabolangelone(15),decursidate(16),(11S,16S,9Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol,1-acetate(17),(11S,16S,9Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol(18),9,12-octadecadienoic acid methyl ester(19),and ligustiphenol(20).Conclusion Compounds 4,8,12,16,17,18 and 20 were firstly isolated from the roots of Angelicae pubescentis.The anti-osteoporosis activity of 20 compounds was screened.Five compounds were found to inhibit RANKL-induced osteoclast differentiation.

关 键 词：独活 香豆素 分离鉴定 破骨细胞 

分 类 号：R284.1[医药卫生—中药学]