初探母源免疫处理草鱼母本及其子代5种免疫因子含量的周年变化规律  

Preliminary study on the annual variation of five immune factors in female parent and offspring of Ctenopharyngodon idella with maternal immunization by grass carp reovirus(GCRV)attenuated vaccine

作　　者：刘益 丁春华 吕钊 余建波[1] 王红权[1] 张学文[1] 石存斌 任燕 王庆 吕丽刚 刘科均 李东放 肖调义[1] 刘巧林[1] LIU Yi;DING Chunhua;LÜZhao;YU Jianbo;WANG Hongquan;ZHANG Xuewen;SHI Cunbin;REN Yan;WANG Qing;LÜLigang;LIU Kejun;LI Dongfang;XIAO Tiaoyi;LIU Qiaolin(Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization,Hunan Agricultural University,Changsha 410128,China;Key Lab of Aquatic Animal Immune Technology of Guangdong Province,Key Lab of Fishery Drug Development of Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China)

机构地区：[1]湖南农业大学,湖南省特色水产资源利用工程技术中心,湖南长沙410128 [2]中国水产科学研究院珠江水产研究所,广东省水产动物免疫技术重点实验室,农业农村部渔用药物创制重点实验室,广东广州510380

出　　处：《水产学报》2022年第12期2396-2408,共13页Journal of Fisheries of China

基　　金：现代农业产业技术体系建设专项(CARS-45-48);湖南省教育厅科学研究重点项目(20A243)。

摘　　要：为探究草鱼母源免疫因子传递特性,实验借助酶联免疫吸附实验(ELISA)和实时荧光定量PCR(qPCR)技术分别检测分析了草鱼呼肠孤病毒(grass carp reovirus,GCRV)弱毒疫苗免疫草鱼母本及其子代(即母源免疫子代)早期胚胎阶段至1龄期间5种免疫因子(IgM、C3、LZM、MBL和Bf)蛋白活性和基因表达水平的周年变化规律。结果显示,草鱼母本经GCRV弱毒疫苗免疫后,血液中5种免疫因子蛋白活性及基因表达水平在产卵期均显著高于对照组1.5倍以上,至免疫后第11个月与对照组无显著差异。母源免疫子代发育至水花(4 d)时,IgM、C3、MBL和Bf的基因表达水平均高于对照组;发育至乌仔(15 d)时,IgM、C3和Bf的蛋白活性水平均略高于对照组;发育至5~11月龄时,5种免疫因子在肝脏、脾脏、肾脏和头肾等免疫器官中仍存在高表达。研究表明,GCRV弱毒疫苗促使母源免疫因子在亲本和子代体内富集表达具有一定的时效性,最长可维持至11个月,为实际生产中草鱼母本疫苗免疫程序的制定提供参考依据。Ctenopharyngodon idella is the most productive cultured freshwater fish species in China,while the healthy development of grass carp industry has been restricted for a long time by grass carp hemorrhage disease caused by grass carp reovirus(GCRV)infection.The selective breeding of C.idella with high resistance is a research focus of industry and academia.Our previous research found that maternal immunization could significantly enhance the offspring resistance to GCRV.To investigate the expression characteristics of maternal immune factors in the female parent treated with GCRV attenuated vaccine and its offspring,the protein concentration and gene expression levels of five different immune factors(IgM,C3,LZM,MBL and Bf)were detected by ELISA and qPCR in the blood of female parent,the early development embryos and juvenile fish of offspring.The results showed that the protein concentration and gene expression level of five immune factors in female parent blood by GCRV attenuated vaccine(experimental group)were 1.5 times higher than those in the control group,but there was no significant difference between the two groups after 11 months of immunization.The gene expression level of IgM,C3,MBL and Bf in experimental group offspring were higher than those of the control group on day 4.The protein concentration level of IgM,C3 and Bf in experimental group offspring were slightly higher than those of the control group on day 15.Five immune factors were still highly expressed in experimental group offspring immune organs such as liver,spleen,kidney and head kidney at the age of 5-11 months.The results indicated that the expression levels of immune factors in the female parent treated with GCRV attenuated vaccine and its offspring significantly increased after maternal immunization by GCRV attenuated vaccine,but it has a certain timeeffectiveness.The high expression of immune factors in the female parents and offspring might last up to 11 months,which provides a reference for the maternal immune procedure in C.idella and

关 键 词：草鱼 草鱼呼肠孤病毒 母源免疫因子 早期发育 时效性 保护效果 

分 类 号：S942.5[农业科学—水产养殖]