基于PCA和隶属函数法分析的马铃薯创新种质抗旱性鉴定与分类  

Identification and classification of drought resistance of potato germplasmbased on PCA and membership function analysis

作　　者：李丰先 罗磊 李亚杰 姚彦红 范奕 李德明 LI Fengxian;LUO Lei;LI Yajie;YAO Yanhong;FAN Yi;LI Deming(Dingxi Academy of Agricultural Sciences,Dingxi 743000;Gansu Province Engineering Research Center for Potato,Dingxi 743000,China)

机构地区：[1]甘肃省定西市农业科学研究院,定西743000 [2]甘肃省马铃薯工程技术研究中心,定西743000

出　　处：《干旱区资源与环境》2022年第11期141-147,共7页Journal of Arid Land Resources and Environment

基　　金：甘肃省寒旱农业项目(GNKJ-2020-1);财政部和农业农村部国家马铃薯产业技术体系(CARS-09);联合国世界粮食计划署甘肃富锌马铃薯小农户试点项目子项目Ⅰ(WFPGSPP-1);甘肃省科技重大专项(GZGG-2021-5);定西市科技计划(DX2022BZ39)资助。

摘　　要：采用盆栽控水法于2020~2021年在智能日光温室对26份马铃薯创新种质的农艺性状、生理生化和产量指标进行测定,用隶属函数法、PCA和系统聚类分析法对马铃薯创新种质抗旱性进行鉴定与分类。结果表明:受到干旱胁迫后,马铃薯创新种质的株高、茎粗、叶片相对含水量和单株结薯数与对照相比均有不同程度的下降,下降幅度小的品系抗旱性较强。叶绿素含量和MDA含量、超氧化物歧化酶(SOD)活性、可溶性糖和脯氨酸含量均有不同程度的增加,增加幅度较大的品系抗旱性较强。经过隶属函数法、PCA和系统聚类分析,筛选出5个抗旱性较强的品系,直接用于参加区域试验或作马铃薯育种遗传材料,为马铃薯种质资源抗旱性鉴定和抗旱型新品种选育提供理论依据。The agronomic traits,physiological and biochemical indexes and yield indexes of 26 potato innovative germplasm were measured by potted water-control method in intelligent solar greenhouse from 2020 to 2021.The drought resistance of potato innovative germplasms was identified and classified by membership function method,PCA and system cluster analysis.The results showed that the plant height,stem diameter,leaf water content and tuber number per plant in innovative potato germplasms decreased in some degrees under drought stress compared with the control,and the strains with smaller decrease had a better drought resistance.Chlorophyll content,MDA content,superoxide dismutase(SOD)activity,soluble sugar and proline content all increased in some degrees too,and the strains with larger increase also had a better drought resistance.Through membership function method,PCA and system cluster analysis,five strains with high drought resistance were screened out,which were directly used in regional test or potato breeding parent materials,to provide theoretical basis for drought resistance identification of potato germplasm resources and breeding of new varieties with drought resistance.

关 键 词：PCA 隶属函数法 生理生化特性 产量 

分 类 号：S532[农业科学—作物学]