施马伦贝格病毒反向遗传系统的研究进展及应用  

Research progress and application of reverse genetic system for Schmallenberg virus

作　　者：韩武玮怡 杨帆[1] 谭金龙 张永芝 崔志伟 宋源富 刘永生 景志忠[1] 李维克 HAN Wu-wei-yi;YANG Fan;TAN Jin-long;ZHANG Yong-zhi;CUI Zhi-wei;SONG Yuan-fu;LIU Yong-sheng;JING Zhi-zhong;LI Wei-ke(Key Laboratory of Veterinary Public Health of the Ministry of Agriculture/State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;Hebei Key Laboratory of Preventive Veterinary Medicine/College of Animal Science and Technology,Hebei Normal University of Science&Technology,Qinhuangdao 066004,China;Baichihe Animal Husbandry and Veterinary Station of Zhucheng City,Weifang 262216,China;Guanzhuang Animal Husbandry and Veterinary Station of Anqiu City,Weifang 262108,China)

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点实验室,甘肃兰州730046 [2]河北科技师范学院动物科技学院河北省预防兽医重点实验室,河北秦皇岛066004 [3]诸城市百尺河畜牧兽医工作站,山东潍坊262216 [4]安丘市官庄畜牧兽医工作站,山东潍坊262108

出　　处：《中国兽医科学》2022年第11期1444-1449,共6页Chinese Veterinary Science

基　　金：甘肃省自然科学基金项目(21JR7RA020);中国农业科学院兰州兽医研究所基本科研业务费项目(1610312021004)。

摘　　要：施马伦贝格病毒(Schmallenberg vir,uSsBV)是一种通过库蠓传播的正布尼亚病毒,由三个负义单链RNA片段(L、M、S片段)组成,以入侵反刍动物中枢神经系统导致其发热、腹泻、产奶减少及胎儿畸形或流产等症状为特征,严重危害地区畜牧业安全,并造成了巨大经济损失。反向遗传操作系统是研究布尼亚病毒的一种有效方法,利用三质粒法拯救出的重组SBV(rSBV)与野生型SBV的生理特性极为相似。为了更深入地了解施马伦贝格病毒及反向遗传操作系统对其重要的作用,本文简要阐述了SBV基本特征、反向遗传操作系统研究进展及反向遗传系统的应用,以期为我国SBV的预防、控制及应用提供参考。Schmallenberg virus(SBV)is an orthobunyavirus transmitted by Culicoides,which consists of three negative single-stranded RNA fragments(L,M,and S fragments)to invade the central nervous system of ruminants resulting in symptoms such as fever,diarrhea,reduced milk production,and fetal malformations or abortion in cattle and sheep,posing a seriously threat to animal husbandry safety and causing significant economic losses.The reverse genetic operating system is a useful tool for studying bunyaviruses,and the physiological characteristics of recombinant SBV(r SBV)rescued using the threeplasmid method are extremely comparable to those of wild-type SBV.In order to understand the important role of the Schmallenberg virus and reverse genetic operating system in SBV,the basic characteristics of SBV,the research progress of the reverse genetic operating system,and the application of the reverse genetic system are briefly described in this paper,to provide a reference for the prevention,control and application of SBV in China.

关 键 词：施马伦贝格病毒 反向遗传操作系统 疫苗 

分 类 号：S852.659.5[农业科学—基础兽医学]