玫瑰花HPLC指纹图谱及质量评价研究  

Study on HPLC Fingerprints and Quality Evaluation of Rosae Rugosae Flos

作　　者：符映均 姜晓燕[1] 张硕 彭敏[1] 张莉 徐容 杨楠[4] 张欣怡 FU Ying-jun;JIANG Xiao-yan;ZHANG Shuo;PENG Min;ZHANG Li;XU Rong;YANG Nan;ZHANG Xin-yi(Department of Pharmacy,Nantong Hospital of Traditional Chinese Medicine,Nantong 226000,China;Chinese Traditional Medicine,Health Food and Cosmetics Laboratory,Jiangsu Institute for Food and Drug Control,Nanjing 210019,China;School of Pharmacy,Nantong Health College of Jiangsu Province,Nantong 226000,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南通市中医院药剂科,江苏南通226000 [2]江苏省食品药品监督检验研究院中药和保健食品化妆品检验室,江苏南京210019 [3]南通卫生高等职业技术学校药学系,江苏南通226000 [4]南京中医药大学药学院,江苏南京210023

出　　处：《现代中药研究与实践》2022年第6期49-54,共6页Research and Practice on Chinese Medicines

基　　金：江苏省卫生健康委科研项目(Z2021081);南通市卫生健康委员会科研项目(QA2021020);南通市“十四五”科教强卫工程青年医学重点人才培养项目。

摘　　要：目的建立玫瑰花的HPLC指纹图谱和没食子酸、原儿茶酸、原花青素B2、鞣花酸及槲皮素的含量测定方法。方法通过正交试验优选玫瑰花中5种指标成分的提取条件,利用HPLC法建立30批玫瑰花酸水解提取物的指纹图谱,进行相似度评价,并对指认的5个成分进行含量测定,使用SPSS 26.0、SIMCA 14.1等软件对实验数据进行分析评价。结果建立了玫瑰花的指纹图谱,共标定20个共有峰。30批样品的相似度均大于0.9,样品差异不大,质量接近,并筛选出对质量影响较大的成分,分别为峰12(鞣花酸)、峰16、峰19(槲皮素)、峰8、峰3、峰6。5种定量成分在一定范围内线性关系良好(r=0.9994~0.9998),平均回收率(n=6)分别为102.15%、97.59%、98.52%、97.38%、98.62%(RSD<2.7%),平均含量分别为6.63 mg/mL、0.57 mg/mL、7.58 mg/mL、19.33 mg/mL、1.48 mg/mL。结论建立的玫瑰花指纹图谱和5个成分含量测定的方法高效、准确、重复性好,可为玫瑰花药材的质量控制、评价提供参考。Objective To establish the method for HPLC fingerprints of Rosae Rugosae Flos and the contents determination method of gallic acid,protocatechuic acid,procyanidin B2,ellagic acid and quercetin.Methods Orthogonal experiment was used to optimal extraction conditions for 5 quantitative components and the fingerprint of the sample was established by the HPLC.Through similarity evaluation,and SPSS 26.0 and SIMCA 14.1 were used to analyze and evaluate the charateristic chromatogram of 30batches of Rosae Rugosae Flos acid hydrolysate.At last,the content of the 5 identified components was also determined.Results The fingerprint of Rosae Rugosae Flos was established,and 20 common peaks were demarcated.The similarities of the each batches of samples were all above 0.9 comparing with the reference,which indicated little differences between the similarities of samples.And the 6 components of peak 12(ellagic acid),peak 16,peak 19(quercetin),peak 8,peak 3 and peak 6 had a greater impact on the quality evaluation.The 5 quantitative components had a good linear relationship with in a certain range(r=0.9994-0.9998),and the average recovery(n=6)were 102.15%,97.59%,98.52%,97.38%,98.62%(RSD<2.7%),which average content were 6.63 mg/mL,0.57 mg/mL,7.58 mg/mL,19.33 mg/mL,1.48 mg/mL,respectively.Conclusion The established fingerprints of Rosae Rugosae Flos and the content determination method are simple,efficient,accurate and repeatable,which can be used for its quality control.

关 键 词：玫瑰花 指纹图谱 质量评价 化学计量分析 

分 类 号：R284.1[医药卫生—中药学]