薰衣草CBF途径相关耐寒基因挖掘与调控网络分析  

Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia

作　　者：蔺海娇 梁雨晨 李玲 马军[2] 张璐 兰振颖 苑泽宁[1] LIN Haijiao;LIANG Yuchen;LI Ling;MA Jun;ZHANG Lu;LAN Zhenying;YUAN Zening(Key Laboratory of Biodiversity of Aquatic Organisms,Harbin Normal University,Harbin 150025,China;Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江省水生生物多样性研究重点实验室,哈尔滨150025 [2]黑龙江省农业科学院经济作物研究所,哈尔滨150086

出　　处：《园艺学报》2023年第1期131-144,共14页Acta Horticulturae Sinica

基　　金：哈尔滨师范大学研究生创新科研项目(HSDSSCX2021-02);黑龙江省政府博士后资助项目(LBH-Z17204);黑龙江省农业科学院青年基金项目(2018YYYF033)。

摘　　要：为探究薰衣草(Lavandula angustifolia Mill.)的耐冷机制,使用转录组学方法对薰衣草响应冷处理的分子调控网络进行挖掘分析。CBF转录因子和其靶基因COR形成的CBF-COR调控网络在增强植物耐冷胁迫中作用显著。本研究中共发现7个CBF基因(LaCBF)和11个COR基因(LaCOR)在冷处理下的表达具有显著差异,同时在CBF信号调控通路中发现4个上游基因LaICE1、LaCAMTA3、LaEBF1、LaCCA1对冷胁迫有响应,认为这些基因共同形成的冷信号调控网络在薰衣草抵御低温中起到了重要作用。To explore the cold-tolerant mechanisms of lavender(Lavandula angustifolia Mill.),transcriptomics methods were used for discovering and analyzing the molecular regulation networks in responses to cold treatments in this research.The CBF-COR regulation networks composed by CBF transcription factors and their target genes COR played significant roles in improving plant cold-tolerance.In this research,seven CBF genes(LaCBF)and 11 COR genes(LaCOR)were found with significant expression difference under cold stress.Meanwhile,four upstream genes including LaICE1,LaCAMTA3,LaEBF1,and LaCCA1 in CBF regulation pathways were found to be responsive to cold stress.It is considered that the cold signal regulation networks together formed by these genes took the key roles in resistance to low temperature in Lavandula angustifolia.

关 键 词：薰衣草 低温胁迫 转录组学分析 CBF转录因子 

分 类 号：S682.3[农业科学—观赏园艺] Q346.7[农业科学—园艺学]