基于比较转录组的延胡索组织差异性表达分析  

Comparative Transcriptome Analysis of Differential Expression in Different Tissues of Corydalis yanhusuo

作　　者：赵雪艳 王琪[2] 王莉 王方圆 王庆[1] 李艳[1] ZHAO Xueyan;WANG Qi;WANG Li;WANG Fangyuan;WANG Qing;LI Yan(Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Xi’an Botanical Garden of Shaanxi Province,Institute of Botany of Shaanxi Province,Xi’an 710061,China;School of Pharmacy,Shaanxi Institute of International Trade and Commerce,Xianyang,Shaanxi 712046,China)

机构地区：[1]陕西省西安植物园,陕西省植物研究所,陕西省植物资源保护与利用工程技术研究中心,西安710061 [2]陕西国际商贸学院医药学院,陕西咸阳712046

出　　处：《园艺学报》2023年第1期177-187,共11页Acta Horticulturae Sinica

基　　金：陕西省自然科学基础研究计划项目(2021JQ-967);陕西省科学院科技计划项目(2020K-28,2021k-17);陕西省教育厅专项科研计划项目(18JK-0953);西安市农业科技研发项目(21NYYF0042,20NYYF0060)。

摘　　要：延胡索(Corydalis yanhusuo)干燥块茎为传统中药,异喹啉生物碱类化合物是其主要的活性成分。采用Illumina高通量测序技术对延胡索块茎、根茎和叶片组织进行转录组测序,鉴定异喹啉生物碱生物合成的相关酶基因,并分析3种组织间的差异表达基因。在块茎vs.根茎、块茎vs.叶、根茎vs.叶比对中分别鉴别到6161、8852和5772个差异表达基因。差异表达基因的KEGG富集分析表明,核糖体、淀粉和蔗糖代谢途径,苯丙素生物合成途径,植物激素信号转导途径以及异喹啉生物碱生物合成途径是主要的富集途径。其中47个转录本与延胡索异喹啉生物碱生物合成相关,其在块茎中的表达量最高,根茎次之,叶中的表达量最低。The dried tubers of Corydalis yanhusuo can be used as the traditional Chinese medicine,and isoquinoline alkaloid compounds are the main active ingredient.To identify genes related to isoquinoline alkaloid biosynthesis and analyze the differentially expressed genes of tuber,rhizome and leaf tissues of C.yanhusuo,transcriptome sequencing of these tissues using Illumina high-throughput sequencing technology were performed.A total of 6161,8852 and 5772 differentially expressed genes were identified in comparisons of tuber vs.rhizome,tuber vs.leaf and rhizome vs.leaf,respectively.KEGG enrichment analysis of differentially expressed genes indicated that major enrichment pathways were starch and sucrose metabolism,phenylpropanine biosynthesis,plant hormone signal transduction and isoquinoline alkaloid biosynthesis pathways.Fourty-seven transcripts were identified to be related to isoquinoline alkaloid biosynthesis and the highest level of gene expression was found in tuber,following by rhizome and leaf.

关 键 词：延胡索 异喹啉生物碱 转录组 表达分析 

分 类 号：S567[农业科学—中草药栽培]