南方大豆品种炭疽病抗性鉴定及抗病相关基因表达分析  

Resistance Identification of Southern Soybean Varieties to Anthracnose and Expression Analysis of Resistance-Related Genes

作　　者：李月 林剑浩 年海[3] 饶军华 周而勋[1] LI Yue;LIN Jian-hao;NIAN Hai;RAO Jun-hua;ZHOU Er-xun(College of Plant Protection,South China Agricultural University/Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Guangzhou 510642,China;Institute of Zoology,Guangdong Academy of Sciences/Guangdong Key Laboratory of Animal Conservation and Resource Utilization/Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Guangzhou 510260,China;College of Agriculture,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学植物保护学院/广东省微生物信号与作物病害防控重点实验室,广东广州510642 [2]广东科学院动物研究所/广东省动物保护与资源利用重点实验室/广东省野生动物保护与利用公共实验室,广东广州510260 [3]华南农业大学农学院,广东广州510642

出　　处：《大豆科学》2023年第1期12-22,共11页Soybean Science

基　　金：广东省重点领域研发计划(2020B020220008)。

摘　　要：为明确南方大豆品种对炭疽病抗性的差异,并分析其抗病相关基因的表达规律,在温室内种植华南农业大学近年来育成和收集到的100份南方春大豆品种,苗期喷洒平头炭疽菌孢子悬浮液,根据叶片病斑面积计算病斑率并划分为6个抗病级别。利用qRT-PCR技术检测典型抗病和感病品种接种平头炭疽菌不同时间后,病程相关蛋白基因、水杨酸途径基因、茉莉酸/乙烯途径基因和多聚半乳糖醛酸酶抑制蛋白基因等抗病相关基因的相对表达量变化情况。结果表明:共鉴定到6份抗病品种、32份中抗品种、57份中感品种和5份感病品种。抗性品种桂1701和感病品种横线早熟豆被平头炭疽菌侵染后抗病基因的表达规律存在很大差异。GmPR2、GmPR4、GmNPR1、GmPAL2-1和GmPAL4在抗病品种的表达量整体较高,GmPR3、GmPR12、GmPAL1-1和GmPAL3-1在感病品种中的表达量整体较高。GmPAL2-1和GmPAL3-1基因在抗病品种中的相对表达量分别在接种后12和6 h极显著升高,而在感病品种中则分别在接种后24和48 h才显著升高。GmPAL2-3在抗病品种中不表达,而在感病品种中表达;GmPGIP2在抗病品种中表达,而在感病品种中不表达。抗病品种中GmLOX7和GmLOX8基因的表达量是感病品种中的400~1 000倍。接种平头炭疽菌后48 h,GmPR2、GmPR4、GmPR5、GmPR10、GmNPR1、GmNAC4和GmPGIP3基因在抗病品种中的相对表达量显著升高,而在感病品种中显著降低。结果显示,桂1701、桂夏1号、南夏豆25、桂夏3号、华夏5和天长小青豆为抗炭疽病大豆品种。不同大豆品种受到平头炭疽菌侵染后,抗感品种间抗病相关基因的表达情况存在较大差异,抗病品种对该病菌侵染的反应更快,推测大豆炭疽菌侵染后48 h是大豆抗病基因响应侵染的关键时间点。In order to clarify the difference of resistance in southern soybean varieties to anthrax, and analyze the expression rule of disease resistance related genes, we planted 100 southern spring soybean varieties bred and collected by South China Agricultural University in recent years in the greenhouse, and sprayed flat ended anthrax spore suspension at seedling stage. We calculated the disease spot rate according to the leaf spot area and divided them into 6 disease resistance levels. We used qRT-PCR to detect the relative expression changes of disease resistance related genes, such as pathogenesis-related protein genes, salicylic acid pathway genes, jasmonic acid/ethylene pathway genes and polygalacturonase inhibitor protein genes, in typical resistant and susceptible varieties inoculated with Colletotrichum truncatum at different times. The results showed that 6 resistant varieties, 32 moderate resistant varieties, 57 moderate susceptible varieties and 5 susceptible varieties were identified. There were significant differences in the expression of disease resistance genes between resistant variety Gui 1701 and susceptible variety Hengxianzaoshudou after being infected by C. truncatum. The expression of GmPR2,GmPR4,GmNPR1,GmPAL2-1 and GmPAL4 were higher in resistant varieties, and GmPR3, GmPR12, GmPAL1-1 and GmPAL3-1 were higher in susceptible varieties. The relative expression of GmPAL2-1 and GmPAL3-1 genes in resistant varieties increased significantly at 12 and 6 hours after inoculation, respectively, while in susceptible varieties, it increased significantly at 24 and 48 hours after inoculation, respectively. GmPAL2-3 was not expressed in resistant varieties, but expressed in susceptible varieties. GmPGIP2 was expressed in resistant varieties, but not in susceptible varieties. The expression of GmLOX7 and GmLOX8 genes in resistant varieties was 400-1 000 times higher than that in susceptible varieties. At 48 hours, the relative expression levels of GmPR2, GmPR4, GmPR5, GmPR10, GmNPR1, GmNAC4 and GmPGIP3 genes

关 键 词：南方大豆 炭疽病 平头炭疽菌 抗病性鉴定 抗病相关基因 表达分析 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]