黄带拟鲹线粒体基因组测序及鲹科鱼类系统发育分析  

Complete mitochondrial genome sequence of Pseudocaranx dentex and phylogenetic analysis of Carangidae

作　　者：王开杰 姜燕[1] 徐永江[1] 柳学周[1] 崔爱君 王滨[1] 薛志勇 毛成全 WANG Kaijie;JIANG Yan;XU Yongjiang;LIU Xuezhou;CUI Aijun;WANG Bin;XUE Zhiyong;MAO Chengquan(Joint Laboratory for Deep Blue Fishery Engineering of Pilot National Laboratory for Marine Science and Technology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao266071,China;National Engineering Research Center For Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,China;Haiyang Yellow Sea Fishery Company,Yantai 265122,China)

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛海洋科学与技术试点国家实验室深蓝渔业工程联合实验室,山东青岛266071 [2]浙江海洋大学,国家海洋设施养殖工程技术研究中心,浙江舟山316022 [3]海阳市黄海水产有限公司,山东烟台265122

出　　处：《水产学报》2022年第11期2017-2027,共11页Journal of Fisheries of China

基　　金：国家重点研发计划(2019YFD0900901,2018YFD0901204);中国水产科学研究院基本科研业务费专项(2020TD47);中国水产科学研究院黄海水产研究所基本科研业务费专项(20603022021011);财政部和农业农村部国家现代农业产业技术体系专项(CARS-47)。

摘　　要：为深入开展黄带拟鲹种质鉴定、分类及遗传进化等研究,通过二代高通量测序与生物信息学分析,揭示了黄带拟鲹线粒体基因组全序列基因结构及鲹科鱼类系统发育特征。结果显示,黄带拟鲹线粒体基因组为典型的环状DNA结构,序列全长为16 570 bp,碱基组成分别为A (27.2%)、G (17.18%)、C (30.24%)和T (25.38%),包括13种蛋白编码基因,22个tRNA基因,2个r RNA基因,除ND6、tRNA^(Gln)、tRNA^(Ala)、tRNA^(Asn)、tRNA^(Cys)、tRNA^(Tyr)、tRNA^(Ser)、tRNA^(Glu)、tRNA^(Pro)外,其余基因均在H链上编码,且基因组存在多处重叠区域。除COⅠ和ND5的起始密码子分别为ATC和ATA外,其余11个蛋白编码基因的起始密码子均为ATG,以典型的TAA和TAG为终止密码子,在Cyt b中存在不完全终止密码子T。黄带拟鲹线粒体基因组全序列与蛋白编码基因的A+T含量分别为52.58%和51.55%,非编码控制区(D-loop) A+T富含61.69%,具有明显的AT偏好性。除tRNA^(Ser)-(GCT)外,其余21个tRNA均含典型三叶草二级结构。为进一步研究黄带拟鲹系统进化特性,通过与18种鲹科鱼类线粒体基因组全序列及16S rRNA基因构建系统进化树显示,黄带拟鲹与高体若鲹同属一个分支,亲缘关系最近。本研究结果可为黄带拟鲹物种鉴别、遗传多样性评价与保护提供技术依据。Pseudocaranx dentex belongs to Actinopterygii,Perciformes,Carangidae,Caranginae,Pseudocaranx and it is widely distributed in the warm waters of Indo-Pacific and Atlantic Ocean.It is mainly distributed in the South China Sea and East China Sea.The research on the breeding of P.dentex in China is still in the initial stage and there are few reports about the genetic background of this species.As a species with high nutritional and economic values,it was studied from the perspective of molecular biology.The complete mitochondrial genome of P dentex was obtained by second-generation sequencing and analyzed through the related software.The results showed that the mitochondrial genome was a typical circular DNA structure,with the total length of the sequence16 570 bp,and the base composition was A (27.2%),G (17.18%),C (30.24%) and T (25.38%).It contained 13 protein-coding genes,22 tRNA genes and 2 rRNA genes.Except for ND6、tRNA^(Gln)、tRNA^(Ala)、tRNA^(Asn)、tRNA^(Cys)、tRNA^(Tyr)、tRNA^(Ser)、tRNA^(Glu)、tRNA^(Pro),all the other genes were encoded on the H-strand.Except for ATC and ATA the starting codon of COⅠand ND5 were ATG;the A+T contents of mitochondrial genome and protein encoding genes were 52.58%and 51.55%respectively,and the noncoding control region (D-loop) is rich in 61.69% which had an obvious A+T bias.Among the 22 tRNA genes,except for tRNA^(ser)-GCT,the other 21 tRNA genes contained typical secondary structure of clover.The phylogenetic tree was constructed with the complete sequence of mitochondrial genome of 18 species belonging to 13 genera of Carangidae fishes.Each species has an independent branch,and P.dentex and Carangoides equula belong to the same branch in the evolutionary tree,which indicated that these two species are closely related to each other.Our findings could help to explore the genetic relationship between P.dentex and other Carangidae fish,and provide a basis for germplasm identification,phylogenetic evolution analysis,genetic diversity evaluation and utilization of P.den

关 键 词：黄带拟鲹 线粒体基因组 序列分析 系统发育 

分 类 号：Q785[生物学—分子生物学] S917.4[农业科学—水产科学]