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作 者:Xuli GAO Shenyang SHEN Qiaohua NIU Weilan MIAO Yuting HAN Ziwei HAO Ning AN Yingyu YANG Yu ZHANG Han ZHANG Kenneth BSTOREY Hui CHANG
机构地区:[1]Shaanxi Key Laboratory for Animal Conservation,Northwest University,Xi'an 710069,China [2]Key Laboratory of Resource Biology and Biotechnology in Western China(College of Life Sciences,Northwest University),Ministry of Education,Xi'an 710069,China [3]Department of Biology,Carleton University,ON K1S 5B6,Canada
出 处:《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2022年第12期1042-1056,共15页浙江大学学报(英文版)B辑(生物医学与生物技术)
基 金:supported by the National Natural Science Foundation of China(Nos.31640072 and 31900338);the Natural Science Basic Research Program of Shaanxi(No.2020JM-428),China.
摘 要:This study compared the effects on bone metabolism and morphology of pathological obesity induced by excessive fat intake in a non-hibernator(mice)versus healthy obesity due to pre-hibernation fattening in a hibernator(ground squirrels).Kunming mice were fed a high-fat diet to provide a model of pathological obesity(OB group).Daurian ground squirrels fattened naturally in their pre-hibernation season(PRE group)were used as a healthy obesity model.Micro-computed tomography(micro-CT)and three-point bending tests were used to determine the microstructure and mechanical properties of bone.Western blots were used to analyze protein expression levels related to bone metabolism(Runt-related transcription factor 2(RunX2),osteocalcin(OCN),alkaline phosphatase(ALP),osteoprotegerin(OPG),receptor activator of nuclear factor-κB ligand(RANKL),cathepsin K,matrix metallopeptidase 9(MMP9),patched protein homolog 1(Ptch1),phosphorylatedβ-catenin(P-β-catenin),and glycogen synthase kinase-3β(GSK-3β)).Compared with controls,there was no obvious bone loss in the OB mice,and the stiffness of the femur was increased significantly.Compared with summer active squirrels,bone formation was enhanced but the mechanical properties did not change in the PRE group squirrels.In OB mice,western blots showed significantly increased expression levels of all proteins except RunX2,OPG,and Ptch1.PRE ground squirrels showed significantly increased expression of most proteins except OCN and Ptch1,which decreased significantly,and P-β-catenin and OPG,which did not change.In conclusion,for non-hibernating mice,moderate obesity had a certain protective effect on bones,demonstrating two-way regulation,increasing both bone loss and bone formation.For pre-hibernating ground squirrels,the healthy obesity acquired before hibernation had a positive effect on the microstructure of bones,and also enhanced the expression levels of proteins related to bone formation,bone resorption,and Wnt signaling.
关 键 词:High-fat diet Pre-hibernation fattening Bone formation Bone loss Wnt signaling
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