sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响  

Effects of soluble glycoprotein 130 on expression of p-STAT3 and vascular endothelial growth factor-A in retina of mice with diabetes mellitus

作　　者：刘光辉[1,2] 史常旋 洪雅军 郑永征[1,2] 王航 孟春[2,3] Guang-Hui Liu;Chang-Xuan Shi;Ya-Jun Hong;Yong-Zheng Zheng;Hang Wang;Chun Meng(Department of Ophthalmology,Affiliated People s Hospital,Fujian University of Traditional Chinese Medicine,Fuzhou 350004,Fujian Province,China;Eye Institute of Integrated Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350004,Fujian Province,China;College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,Fujian Province,China)

机构地区：[1]福建中医药大学附属人民医院眼科,福建省福州市350004 [2]福建中医药大学中西医结合眼科研究所,福建省福州市350004 [3]福州大学生物科学与工程学院,福建省福州市350108

出　　处：《国际眼科杂志》2023年第3期375-378,共4页International Eye Science

基　　金：国家自然科学基金面上项目(No.81774369);福建省卫生健康中青年科研重大项目(No.2021ZQNZD012)。

摘　　要：目的:观察可溶性糖蛋白130(sgp130)对糖尿病(DM)小鼠视网膜p-STAT3及VEGF-A表达的影响,探讨sgp130防治糖尿病视网膜病变(DR)炎性损害的可能性。方法:小鼠45只被随机分为正常组、DM组和sgp130组。采用链脲佐菌素对DM组和sgp130组进行DM造模。正常组、DM组不做特殊干预,sgp130组在第1、5wk被给予1.5mg/mL sgp1302μL进行玻璃体腔注射治疗。10wk后处死所有小鼠,检查视网膜组织IL-6、p-STAT3、VEGF-A等蛋白的表达。结果:在第10wk时,DM组视网膜IL-6、p-STAT3、VEGF-A表达较正常组升高(均P<0.01)。sgp130组p-STAT3、VEGF-A表达水平较DM组低(均P<0.01)。结论:sgp130可以选择性拮抗IL-6反式信号传导通路,下调下游炎性因子VEGF-A的表达,可以用于干预DM引起的IL-6相关性视网膜炎性损害。·AIM:To observe the effect of soluble glycoprotein 130(sgp130) on expression of p-STAT3 and vascular endothelial growth factor(VEGF)-A in retina of mice with diabetes mellitus(DM),and explore the possibility of sgp130 in interfering with inflammatory damage of diabetic retinopathy(DR).·METHODS:A total of 45 mice were randomly divided into normal group,DM group and sgp130 group.DM models were made in DM group and sgp130 group with streptozotocin.No special intervention was given to normal group and DM group,but sgp130 group was given intravitreal injection of 1.5mg/mL sgp130 2μL at the 1 and 5wk.After 10wk,all the mice were sacrificed to assess the protein expression of interleukin 6(IL-6),p-STAT3 and VEGF-A in the retina.·RESULTS:The expressions of IL-6,p-STAT3 and VEGF-A in retina of DM group were higher than those of normal group at 10wk(all P<0.01).The expression of p-STAT3 and VEGF-A in sgp130 group were lower than those in DM group(all P<0.01).·CONCLUSION:The sgp130 can selectively antagonize the trans signal transduction pathway of IL-6,down-regulate the expression of downstream inflammatory factors VEGF-A,and it may be used in the intervention of retinal inflammatory damage related with IL-6 in DM.

关 键 词：糖尿病视网膜病变 炎症 白介素6 P-STAT3 血管内皮生长因子A(VEGF-A) 可溶性糖蛋白130(sgp130) 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]