雌激素受体基因多态性与拜城油鸡繁殖性能的关联性分析  

Association analysis between polymorphisms of the ESR gene and production performance of Baicheng Oil chicken

作　　者：丁梦琴 胡雅丽 黑达西·巴哈提 彭凤强 沙尔山别克·阿不地力大[1] DING Mengqin;HU Yali;HEIDAXI Bahati;PENG Fengqiang;SHAERSHANBIEKE Abudilida(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物科学学院,新疆乌鲁木齐830052

出　　处：《畜牧与兽医》2023年第2期18-24,共7页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31460584)。

摘　　要：旨在探究雌激素受体(ESR)基因多态性与拜城油鸡产蛋性状、蛋品质及孵化性能的关系,为高生产性能种鸡的选育提供新的分子标记。试验以129只拜城油鸡为研究对象,利用DNA混池重测序技术筛选SNPs位点,采取Sequenom飞行时间质谱技术进行相关的位点分型,通过SPSS 26.0进行繁殖性能关联分析。结果显示:所筛选出的6个ESR基因SNPs位点分别为g.48747211 T>G、g.48777663 C>T、g.48817503 G>A、g.52521976 T>C、g.52529021 A>G和g.52541181 C>T,且均存在3种基因型,哈代-温伯格平衡检验表明6个SNPs位点均处在平衡状态,其中g.48747211 T>G、g.48777663 C>T、g.52521976 T>C和g.52541181 C>T位点多态信息含量均大于0.25,处于中度多态。关联性分析表明,ESR1基因g.48747211 T>G和g.48777663 C>T位点与拜城油鸡300日龄总产蛋量存在极显著相关性(P<0.01),纯合基因型GG 300日龄总产蛋量极显著高于基因型GT,纯合基因型TT 300日龄总产蛋量极显著高于基因型CT,杂合基因型GT和CT个体的300日龄总产蛋量最低。ESR2基因g.52521976 T>C和g.52529021 A>G位点与拜城油鸡入孵蛋孵化率和入孵蛋死胚率显著相关(P<0.05),与受精蛋孵化率极显著相关(P<0.01),其中纯合基因型CC和TT的个体入孵蛋孵化率显著高于杂合基因型CT,CC和TT基因型的个体受精蛋孵化率极显著高于基因型CT,CC和TT基因型的入孵蛋死胚率显著低于基因型CT。综上,ESR1基因与拜城油鸡300日龄产蛋量密切相关,ESR2基因与拜城油鸡孵化性能具有紧密关联性。In order to explore the relationship between the polymorphisms of the ESR gene and the egg production traits,egg quality and hatching performance of Baicheng Oil chickens,new molecular markers were provided for the rearing of breeding chickens with high production performance.The experiment had 129 Baicheng Oil chickens as the research subjects.The DNA mixed pool resequencing technology was sued to screen SNPs.The Sequenom time-of-flight mass spectrometry technology was used for related site typing,and SPSS 26.0 was used for reproductive performance correlation analysis.The results showed that the six ESR gene SNPs were g.48747211 T>G,g.48777663 C>T,g.48817503 G>A,g.52521976 T>C,g.52529021 A>G and g.52541181 C>T;and all presented 3 genotypes.The Hardy-Weinberg equilibrium test showed that 6 SNPs loci were in equilibrium,of which the polymorphic information content of the g.48747211 T>G.g.48777663 C>T,g.52521976 T>C and g.52541181 C>T sites was greater than 0.25,a moderate polymorphism.Correlation analysis showed that the g.48747211 T>G and g.48777663 C>T sites of the ESR1 gene were significantly correlated with the total egg production of Baicheng Oil chickens at 300 days of age(P<0.01),the total egg production of the homozygous genotype GG of these chickens was significantly higher than that of genotype GT;the total egg production of their homozygous genotype TT was significantly higher than that of genotype CT,and the total egg production of heterozygous genotype GT and CT of these individuals was the lowest.The g.52521976 T>C and g.52529021 A>G loci of the ESR2 gene were significantly correlated with the incubation rate and the dead embryo rate of incubated eggs of Baicheng Oil chickens(P<0.05),and the incubation rate of fertilized eggs was significantly correlated with the fertilized egg hatching rate(P<0.01)The hatching rate of individual incubated eggs with CC and TT was significantly higher than that of heterozygous genotype CT,the incubation rate of individual fertilized eggs in CC and TT genotypes was si

关 键 词：雌激素受体 SNPS 拜城油鸡 生产性能 关联性 

分 类 号：S831.3[农业科学—畜牧学]