磷酸化乌兹别克甘草多糖的体外免疫增强活性研究  

Study on the immune enhancement of phosphorylated glycyrrhiza polysaccharide in vitro

作　　者：韩健宝 仇天新 衣蕾[1] 吴昱 徐孝宙 武毅[1] 胡元亮[1] HAN Jianbao;QIU Tianxin;YI Lei;WU Yu;XU Xiaozhou;WU Yi;HU Yuanliang(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《畜牧与兽医》2023年第2期110-116,共7页Animal Husbandry & Veterinary Medicine

摘　　要：为了探究磷酸化乌兹别克甘草多糖(pGPS)的免疫增强作用,以鸡外周血T淋巴细胞和小鼠骨髓源树突状细胞为靶细胞,用MTT法和形态观察法测定了pGPS对鸡外周血T淋巴细胞增殖、对小鼠树突状细胞分化和增殖以及对淋巴细胞和树突状细胞细胞因子分泌的影响。结果显示:对于鸡外周血T淋巴细胞,在39.062~625μg/mL浓度的pGPS单独刺激时,淋巴细胞增殖率显著高于细胞对照组(P<0.05);在78.125~156.25μg/mL的pGPS与植物血凝素(PHA)共同刺激时,淋巴细胞增殖率显著高于PHA对照组(P<0.05);pGPS在39.062~312.5μg/mL时显著增加淋巴细胞分泌的干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、白介素6(IL-6)(P<0.05)。对于小鼠树突状细胞,pGPS组树突状细胞的刺突明显多于空白组;pGPS在0.977~15.625μg/mL时,未成熟树突细胞的增殖率显著高于脂多糖(LPS)对照组,并且显著促进树突状细胞IFN-γ和TNF-α的分泌(P<0.05)。结果表明:pGPS在合适的浓度能单独或协同PHA显著促进外周血T淋巴细胞增殖和细胞因子分泌,显著促进未成熟树突状细胞分化、增殖和细胞因子分泌,从而增强免疫。In order to investigate the immune-enhancing effects of phosphorylated glycyrrhiza polysaccharide(pGPS),peripheral-derived T cells from chickens and bone marrow-derived dendritic cells(DCs)from mice were obtained,cultured and activated.The proliferation of the cells was detected by MTT assay and the morphology of DCs was examined.The results revealed that the lymphocyte proliferation rate of the pGPS(39.062-625μg/mL)was significantly higher than that of the cells of the control group(P<0.05).After co-stimulated with PHA,the lymphocyte proliferation rate in the pGPS(78.125-156.25μg/mL)group was significantly higher than that in the PHA group(P<0.05).pGPS significantly increased the secretion of IFN-γ,TNF-αand IL-6 by lymphocytes at 39.062-312.5μg/mL(P<0.05).For the murine DCs,after stimulated by pGPS for 7 days,the podosomes of DCs were more abundant than that in the cells of the control group.When the concentration of pGPS was in the range of 0.977-15.625μg/mL,the proliferation rate of the immature DCs was significantly higher than that of the LPS control group,and significantly promoted the secretion of DCs IFN-γand TNF-α(P<0.05).The present results indicated that pGPS significantly enhanced the proliferation of peripherial lymphocytes alone with or without PHA under the appropriate concentration,and promoted the cytokines to be secreted by lymphocytes and DCs.In addition,the differentiation and proliferation of immature DCs were markedly enhanced by pGPS,thus enhancing immunity.

关 键 词：磷酸化甘草多糖 外周血T淋巴细胞 骨髓源树突状细胞 增殖 

分 类 号：S859.3[农业科学—临床兽医学]