基于肿瘤诊疗一体化纳米药物载体的构建及性能研究  

Construction and Performance Study of Nano Drug Carriers based on the Integration of Tumor Diagnosis and Treatment

作　　者：李海洋 陈泽坤 颜瑞 陶彩虹[1] LI Hai-yang;CHEN Ze-kun;YAN Rui;TAO Cai-hong(School of Chemistry and Chemical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)

机构地区：[1]兰州交通大学化学化工学院,兰州730070

出　　处：《兰州交通大学学报》2023年第1期127-138,共12页Journal of Lanzhou Jiaotong University

基　　金：甘肃省自然科学基金(18JR3RA109);甘肃省高等学校创新基金项目(2022A-043);兰州交通大学“百优人才”青年人才计划项目。

摘　　要：癌症诊疗一体化复合材料有望将诊断和治疗进行协同作用,通过设计将具有肿瘤诊断和治疗功能的组分组装在一个纳米体系中,实现对肿瘤的早期诊断、靶向定位和精准治疗.采用溶剂热法制备了Fe3O4纳米颗粒作为内核,采用自组装法将介孔聚多巴胺(MPDA)构筑在Fe3O4内核表面(以Pluronic F127和1,3,5-三甲基苯(TMB)为模板剂).随后,采用原位沉积法,在MPDA层上修饰Au纳米粒子和亚甲基蓝(MB)荧光染料,并装载抗癌药物盐酸阿霉素(DOX).最后,用牛血清蛋白(BSA)包覆上述复合粒子,得到纳米药物载体Fe3O4@MPDA@Au/MB/DOX@BSA粒子.对MB染料的吸附性能、 DOX的负载和释放性能、纳米载药体的光热性能以及体外细胞毒性进行了研究.结果表明:所制备的纳米药物载体具有较高的光热转换效率和良好的生物相容性;该载体24 h内平均DOX负载量为96.26 mg/g, 8 h内在酸性和近红外光(NIR)条件下DOX的释放率约为50.9%.Fe3O4@MPDA@MB/Au/DOX@BSA纳米药物载体集化疗与光热治疗于一体,可对肿瘤细胞的生长进行有效抑制.The integrated cancer diagnosis and therapy composite is expected to bring diagnosis and treatment together.By integrating components with tumor diagnosis and treatment functions into a nano-system, early diagnosis, targeted localization and precise therapy of tumors can be realized.In this paper, Fe3O4nanoparticleswas prepared as the core by the solvothermal method.Drug carriers with mesoporous polydopamine(MPDA)as the shell layer were prepared by the self-assembly method(mesoporous were obtained from Pluronic F127 and 1,3,5-Trimethylbenzene(TMB) as template agents and washed away by solvent).Subsequently, Au nanoparticles were modified on MPDA layer by in situ deposition;and methylene blue(MB) fluorescent dye was also modified on MPDA surface;and the anti-cancer drug adriamycin hydrochloride(DOX) was loaded.Finally, Fe3O4@MPDA@Au/MB/DOX@BSA nanodrug carriers were prepared by encapsulating above composite particles with bovine serum protein(BSA).The adsorption properties, DOX loading and release properties, photothermal properties and in vitro cytotoxicity of MB dyes were investigated.The results showed that the prepared nano drug carriers had high photothermal conversion efficiency and good biocompatibility.The average DOX loading of the prepared carriers was 96.26 mg/g within 24 h, and the release rate was about 50.9% under acid and photothermal conditions within 8 h.The preparation process of Fe3O4@MPDA@MB/Au/DOX@BSA nanodrug carriers combined chemotherapy and photothermal therapy to achieve significant inhibition of tumor growth.

关 键 词：介孔聚多巴胺 金纳米粒子 联合治疗 诊疗一体化 纳米药物载体 

分 类 号：O61[理学—无机化学]