miRNA-34a和癌胚抗原在良恶性胸腔积液鉴别诊断中的应用价值  

Application value of miRNA-34a and carcinoembryonic antigen in differential diagnosis of benign and malignant pleural effusion

作　　者：周黎[1] 黄伟萍[1] 王世俊[1] 陈捷[1] ZHOU Li;HUANG Wei-ping;WANG Shi-jun;CHEN Jie(Yuyao People's Hospital,Zhejiang 315400,China)

机构地区：[1]浙江省余姚市人民医院,浙江315400

出　　处：《中国卫生检验杂志》2023年第3期320-322,331,共4页Chinese Journal of Health Laboratory Technology

基　　金：余姚市科技计划项目(2020YZD02)。

摘　　要：目的 探讨miRNA-34a和癌胚抗原(CEA)的检测在良恶性胸腔积液鉴别诊断中的应用价值。方法 收集良恶性胸腔积液患者各35例,采用实时荧光定量PCR测定miRNA-34a的相对表达量,全自动免疫分析仪测定血清CEA水平,应用ROC曲线评估miRNA-34a和CEA的诊断性能。结果 恶性胸腔积液组患者miRNA-34a相对表达量低于良性胸腔积液组,差异有统计学意义(P<0.01);恶性胸腔积液组患者CEA浓度高于良性胸腔积液组,差异有统计学意义(P<0.01)。ROC曲线分析,miRNA-34a和CEA诊断恶性胸腔积液曲线下面积(AUC)分别为0.704和0.764。结论 miRNA-34a在恶性胸腔积液中呈低表达,可能与相关肿瘤的胸膜转移有关。胸腔积液中miRNA-34a和CEA检测对恶性胸腔积液的诊断具有一定的辅助性价值。Objective This paper aim to explore the application value of miRNA-34a and carcinoembryonic antigen(CEA) in differential diagnosis of benign and malignant pleural effusion. Methods A total of 35 cases with benign and malignant pleural effusion were collected, the relative expression of miRNA-34a was determined by real-time fluorescence quantitative PCR, the serum CEA level was determined by automatic immunoassay analyzer, and the diagnostic performance of miRNA-34a and CEA was evaluated by ROC curve. Results The relative expression of miRNA-34a in the malignant pleural effusion group was significantly lower than that in the benign pleural effusion group, and the difference was statistically significant(P<0.01). The concentration of CEA in the malignant pleural effusion group was significantly higher than that in the benign pleural effusion group, and the difference was statistically significant(P<0.01). ROC curve analysis showed that the area under the curve(AUC) of miRNA-34a and CEA in the diagnosis of malignant pleural effusion were 0.704 and 0.764, respectively. Conclu sion The low expression of miRNA-34a in malignant pleural effusion may be related to pleural metastasis of related tumors. The detection of miRNA-34a and CEA in pleural effusion has certain auxiliary value for the diagnosis of malignant pleural effusion.

关 键 词：miRNA-34a 癌胚抗原 胸腔积液 应用价值 

分 类 号：R561.3[医药卫生—呼吸系统]