不同温度处理对乳苣种子萌发生理生化的影响  

Effects of different temperature treatments on physiological and biochemical characteristics of Lactuca tatarica during seed germination

作　　者：卢旭东 张凤兰[1] 杨忠仁[1] 刘金泉[1] 郝丽珍[1] 伊风艳 LU Xudong;ZHANG Fenlang;YANG Zhongren;LIU Jinquan;HAO Lizhen;YI Fengyan(Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resources and Germplasm Enhancement,College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010019;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室,呼和浩特010019 [2]内蒙古自治区农牧业科学院,呼和浩特010031

出　　处：《安徽农业大学学报》2022年第6期913-919,共7页Journal of Anhui Agricultural University

基　　金：内蒙古自治区应用技术研究与开发项目(2019GC237);内蒙古自治区科技成果转化专项资金项目(2021CG0023)生态草业可持续发展研究专项(2022CYZX05);内蒙古农牧业科学院创新基金项目(2019CXJJN11);内蒙古农牧业科学院青年创新基金项目(2020QNJJN012)共同资助。

摘　　要：为了解温度对乳苣种子萌发以及种子萌发过程中贮藏物质和各种酶的变化情况,开展了不同温度对乳苣种子萌发以及贮藏物质变化规律的研究。结果表明:变温能够有效提高乳苣种子的发芽率,最适变温为15/30℃,发芽率可达54.45%;乳苣种子萌发过程中,种子内含物质利用在时间上存在差异,碳水化合物动员较快,脂肪、蛋白质水解相对较慢。乳苣种子萌发过程中,其碳水化合物的代谢主要归因于α-淀粉酶的水解,且α-淀粉酶的活性与种子萌发率呈正相关;乳苣种子萌发过程中,TCA及PPP同时存在,PPP对乳苣种子萌发起主导地位。The paper studied the changes of seed germination and storage material at different temperature to understand the changes of storage material and various enzymes during the germination of Lactuca tatarica seeds.The results showed that:variable temperature could increase the germination rate of seeds.When the optimum variable temperature was 15 ℃ or 30 ℃,the highest germination rate of the seeds could reach to 54.45%;during the seed germination,the nutrients were gradually hydrolyzed under the action of related enzymes,which provided nitrogen source,carbon source and energy for the growth of embryo.There were some differences in the degradation process of the substance in the seeds.Among them,carbohydrate mobilization was fast,while the hydrolysis of fat and protein was relatively slow.During the germination of L.tatarica,the carbohydrate metabolism mainly attributes to the hydrolysis of α-amylase,and its enzyme activity is positively correlated with the germination rate of seeds;tricarboxylic acid cycle(TCA) and pentose phosphate pathway(PPP) coexist during seed germination of L.tatarica,and PPP plays a dominant role in seed germination of L.tatarica.

关 键 词：乳苣 萌发 贮藏物质 酶活性 

分 类 号：S647[农业科学—蔬菜学]