肺原位移植瘤小鼠模型的建立  

Establishment of an orthotopic lung cancer model

作　　者：杨星九 张文龙 史旭东[1,3] 李梦媛 张国新[1,4] 高苒 YANG Xingjiu;ZHANG Wenlong;SHI Xudong;LI Mengyuan;ZHANG Guoxin;GAO Ran(Institute of Laboratory Animal Sciences,Chinese Academy of Medical Science(CAMS),Comparative Medicine Center,Peking Union Medical College(PUMC),Beijing 100021,China;National Human Disease Animal Model Resource Center,Beijing 100021;NHC Key Laboratory of Human Disease Comparative Medicine,Beijing 100021;Beijing Engineering Research Center for Experimental Animal Models of Human Critical Disease,Beijing 100021)

机构地区：[1]中国医学科学院医学实验动物研究所北京协和医学院比较医学中心,北京100021 [2]国家人类疾病动物模型资源库,北京100021 [3]国家卫生健康委员会人类疾病比较医学重点实验室,北京100021 [4]北京市人类重大疾病实验动物模型工程技术研究中心,北京100021

出　　处：《中国实验动物学报》2022年第8期1064-1070,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-013)。

摘　　要：目的本研究拟建立一种小鼠肺癌原位移植瘤模型,用于模拟临床患者肺癌的发生及发展。方法构建表达荧光素酶报告基因的小鼠肺癌细胞LLC-luc,通过手术将LLC-luc细胞注射至小鼠左肺,构建原位移植瘤模型。使用CT和活体荧光成像系统对原位移植瘤模型进行示踪监测,并于肿瘤移植后第14天取材,IHC检测组织免疫学及免疫微环境特征。结果LLC-luc细胞注射后,原位移植肿瘤成功定植于左肺,可通过CT和活体荧光成像示踪监测肿瘤的发展过程,IHC结果显示肿瘤具备转移潜质,肿瘤内部存在免疫细胞浸润。结论本研究成功建立了一种易于评价,贴近于临床患者肺癌的发展的小鼠肺癌原位移植瘤模型。Objective A mouse orthotopic transplanted tumor model of lung cancer was established to simulate the occurrence and development of lung cancer in clinical patients.Methods The LLC cell line expressing a luciferase reporter gene was established(LLC-luc).LLC-luc cells were injected into the left lung of mice to establish an orthotopic tumor model.CT and in vivo fluorescence imaging were used for tracer monitoring of the orthotopic tumor model.Lung tumors were collected on day 14 after cell injection.The characteristics of tissue immunology and the immune microenvironment were assessed by IHC.Results After LLC-luc cell injection,a tumor had formed in the left lung.The tumor has a metastatic potential and immune cell infiltration was detected.Conclusions We successfully established a mouse model of orthotopic lung cancer,which is simple to evaluate and mimics the development of lung cancer in clinical patients.

关 键 词：肺癌 原位 移植瘤模型 影像学 

分 类 号：Q95-33[生物学—动物学]