Tn5转座子及其在生物技术领域的应用  

Tn5 transposon and its applications in biotechnology

作　　者：高畅 任鹏程 李明格 吕赫喆 胡仪[1] GAO Chang;REN Pengcheng;LI Mingge;LYU Hezhe;HU Yi(College of Life Sciences,Beijing Normal University,Beijing 100875,China;Institute of Zoology,Chinese Academy of Sciences,Beijing 100101)

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]中国科学院动物研究所,北京100101

出　　处：《中国实验动物学报》2022年第8期1087-1094,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(32070401)。

摘　　要：随着二代测序技术的快速发展,测序及其相关技术愈发成熟并向高精度和高通量的方向深入。传统的测序方式对样本量要求很高,而许多珍贵样本难以满足要求,因此能够降低样本量需求的技术亟待开发。而Tn5转座酶在文库构建中可以在片段化DNA的同时将接头序列加入到靶序列两侧,大大缩减了实验流程,同时也降低了对样本量的需求。因此,Tn5转座酶在生物技术领域的应用被逐渐开发,例如CUT&Tag、stLFR和TRACE-seq等技术就是将Tn5转座酶应用于表观测序、长片段测序和转录组测序等领域的研究成果。简单的实验流程和极低的成本使其备受青睐。本文中,我们对Tn5转座子及其在生物技术领域的应用进行了综述,以期方便大家理解Tn5转座子及其相关技术,为未来Tn5转座子相关技术的改进和推广提供参考。With the rapid advancement of next-generation sequencing(NGS),sequencing and its related technologies are becoming more mature and providing higher accuracy and throughput.Traditional method of sequencing require a large sample quantity that is difficult to obtain for some samples.Thus,techniques with a lower demand on sample quantity are in urgent need.In library construction,the Tn5 transposase cuts DNA and adds an adapter sequence to both sides of the target sequence,which requires fewer steps and a lower sample quantity.Therefore,applications of Tn5 transposase have been gradually developed in biotechnology.For example,CUT&Tag,stLFR,and TRACE-seq are techniques that use Tn5 transposase in epigenomic sequencing,long fragment reading,and transcriptome sequencing.The simple steps and very low cost make the techniques popular.In this review,we summarize the Tn5 transposon and its applications in biotechnology to make it understood more easily and provide a reference for improvement and promotion in the future.

关 键 词：二代测序 Tn5转座子 生物技术 

分 类 号：Q95-33[生物学—动物学]