光声成像技术定量评估大鼠骨骼肌钝挫伤模型血氧变化的可行性  

Feasibility of photoacoustic imaging technique for quantitative evaluation of blood oxygen saturation changes in a rat skeletal muscle blunt contusion model

作　　者：刁宗平 赵佳琦 汤晓轶 DIAO Zong-ping;ZHAO Jia-qi;TANG Xiao-yi(Department of Ultrasound,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Department of Ultrasound,Shanghai Fourth People’s Hospital Affiliated to Tongji University,Shanghai 200434,China;Department of Ultrasound,The Second Affiliated Hospital of Naval Medical University(Second Military Medical University),Shanghai 200003,China)

机构地区：[1]上海交通大学医学院附属瑞金医院超声科,上海200025 [2]同济大学附属上海市第四人民医院超声医学科,上海200434 [3]海军军医大学(第二军医大学)第二附属医院超声诊断科,上海200003

出　　处：《海军军医大学学报》2023年第2期170-177,共8页Academic Journal of Naval Medical University

基　　金：国家自然科学基金(81501492);上海市自然科学基金(20ZR1457900);海军军医大学(第二军医大学)第二附属医院人才建设三年行动计划——“金字塔”人才工程军事医学人才项目(1009)。

摘　　要：目的探讨光声成像技术定量评估钝挫伤受损骨骼肌血氧变化的可行性。方法将25只雄性SD大鼠随机分为5组,正常对照组及损伤后1、2、3、7 d组各5只。各损伤组大鼠通过重物打击右后肢建立骨骼肌钝挫伤模型,正常对照组大鼠不做任何处理。应用彩色多普勒超声成像(CDFI)观察损伤组大鼠各时间点受损区域和正常对照组相同部位骨骼肌的血流状况,并进行血流Alder半定量分级;同步启动光声成像模式,检测感兴趣区域的平均血氧饱和度和总血氧饱和度。采用Fisher精确概率法分析CDFI血流Alder半定量分级与平均血氧饱和度和总血氧饱和度的相关性。结果正常对照组及损伤后1、2、3、7 d组大鼠骨骼肌CDFI血流Alder半定量分级分别为Ⅰ、0~Ⅰ、Ⅱ、Ⅱ~Ⅲ、Ⅲ级。损伤后2、3、7 d组骨骼肌的平均血氧饱和度、总血氧饱和度高于正常对照组与损伤后1 d组(P均<0.05),损伤后7 d组均高于损伤后2、3 d组(P均<0.05),而损伤后2 d组与3 d组之间差异无统计学意义(P均>0.05)。25只大鼠中CDFI血流Alder半定量分级为0~Ⅰ级10只、Ⅱ~Ⅲ级15只。平均血氧饱和度<55%的大鼠11只,其中CDFI血流Alder半定量分级为0~Ⅰ级8只;≥55%的大鼠14只,其中CDFI血流Alder半定量分级为Ⅱ~Ⅲ级12只。总血氧饱和度<55%的大鼠11只,其中CDFI血流Alder半定量分级为0~Ⅰ级9只;≥55%的大鼠14只,其中CDFI血流Alder半定量分级为Ⅱ~Ⅲ级13只。CDFI血流Alder半定量分级与平均血氧饱和度和总血氧饱和度均呈正相关(P=0.005、0.002)。结论光声成像可动态评估钝挫伤骨骼肌不同时期的血氧饱和度变化,且与CDFI血流Alder半定量分级相关性较好。Objective To investigate the feasibility of photoacoustic imaging for quantitative evaluation of blood oxgen saturation changes in skeletal muscle injured by blunt contusion.Methods Twenty-five SD rats were randomly divided into 5 groups:normal control group and 1,2,3 and 7 d post-injury groups,with 5 rats in each group.The skeletal muscle blunt contusion model was established by striking the right hind limb with a heavy object in each injury groups,and the rats in the normal control group were not given any treatment.Color Doppler flow imaging(CDFI)was used to observe the blood supply of the skeletal muscle of injured area at each time point in the injury groups and the corresponding sites in the normal control group,and Alder semi-quantitative grading was used to record the blood supply parameters of each group.The photoacoustic imaging mode was performed to measure the average blood oxygen saturation and the total blood oxygen saturation within each region of interest.The correlations between Alder semi-quantitative grading of CDFI and average blood oxygen saturation and total blood oxygen saturation were analyzed by Fisher exact test.Results The CDFI blood flow Alder semi-quantitative grades of skeletal muscle in the normal control group and 1,2,3 and 7 d post-injury groups wereⅠ,0-Ⅰ,Ⅱ,Ⅱ-ⅢandⅢ,respectively.The average blood oxygen saturation and total blood oxygen saturation in the 2,3 and 7 d post-injury groups were significantly higher than those in the normal control group and the 1 d post-injury group(all P<0.05),and those in the 7 d post-injury group were significantly higher than the 2 and 3 d post-injury groups(all P<0.05),while there was no statistical significance between the 2 and 3 d post-injury groups(both P>0.05).The CDFI blood flow Alder semi-quantitative grading results were:grades 0 toⅠin 10 and gradesⅡtoⅢin 15 of 25 rats.There were 11 rats with average blood oxygen saturation<55%,including 8 rats with grades 0 toⅠ;and 14 rats with average blood oxygen saturation≥55%,incl

关 键 词：光声成像 彩色多普勒血流成像 骨骼肌 挫伤 血氧饱和度 

分 类 号：R445.1[医药卫生—影像医学与核医学] R685[医药卫生—诊断学]