UVB照射后背根神经节水平CCL2对外周痛觉调控的作用研究  

Effects of CCL2 in dorsal root ganglion on the regulation of peripheral pain after UVB irradiation

作　　者：胡杨 申美伦 周怡民 杜尚锐 韩倩 郭莹 邢俊玲 HU Yang;SHEN Meilun;ZHOU Yimin;DU Shangrui;HAN Qian;GUO Ying;XING Junling(Department of Radiation Medical Protection,School of Military Preventive Medicine;Department of Radiation Medical Protection,No.3 Cadet Regiment,School of Basic Medical Sciences;Department of Radiation Medical Protection,No.5 Cadet Regiment,School of Basic Medical Sciences,Air Force Medical University,Xi an,710032,China)

机构地区：[1]空军军医大学军事预防医学系辐射防护医学教研室,陕西西安710032 [2]空军军医大学基础医学院学员三大队,陕西西安710032 [3]空军军医大学基础医学院学员五大队,陕西西安710032

出　　处：《空军军医大学学报》2023年第2期123-129,134,共8页Journal of Air Force Medical University

基　　金：国家自然科学基金(31671089)。

摘　　要：目的研究紫外线B段(UVB)照射后背根神经节(DRG)内趋化因子CC配体2(CCL2)对机械痛敏形成的影响,探讨UVB所致痛敏中CCL2对卫星胶质细胞Kir 4.1通道的调控作用。方法小鼠UVB单次足底照射造模后进行痛行为检测,之后分别在4个时间点(1、2、4、7 d)取DRG进行CCL2及Kir 4.1表达的测定。另取UVB造模后2 d的动物,随机分为对照组、Bindarit组、CCL2组。Bindarit组和CCL2组分别在DRG注射Bindarit和CCL2,第2日取材后,3组动物分别进行CCL2及Kir 4.1表达的测定。结果与照射前相比,UVB照射后机械痛阈值显著降低(P<0.01),第2日降至最低,第14日恢复正常;UVB造模后,CCL2和Kir 4.1均出现显著上调(P<0.01)后逐渐降低的变化过程。DRG内注射Bindarit阻断CCL2合成后,Kir 4.1显著下调(P<0.01),注射CCL2增加CCL2含量后,Kir 4.1显著上调(P<0.05)。结论UVB照射后外周痛觉敏化的产生与DRG内CCL2的上调有关,而继发于CCL2变化的Kir 4.1的上调可能起到抑制神经元兴奋性增高的作用。Objective To investigate the effects of CCL2 in dorsal root ganglion(DRG) on the formation of mechanical hyperalgesia after ultraviolet B(UVB) irradiation, and to explore the regulatory effect of CCL2 on Kir 4.1 channel in satellite glial cells in UVB-induced hyperalgesia. Methods After UVB modeling, pain behavior of mice was detected, and then DRG was taken at 4 time points(1, 2, 4, 7 d) to detect the expression of CCL2 and Kir 4.1. Two days after UVB modeling, animals were randomly divided into 2 d control group, Bindarit group and CCL2 group, in which Bindarit group and CCL2 group were injected with Bindarit and CCL2 in DRG, respectively. After sampling on the second day, the expressions of CCL2 and Kir 4.1 were measured in the three groups, respectively. Results Compared with the control group, the mechanical pain threshold was significantly decreased after UVB irradiation(P<0.01), reaching its lowest point on the second day and recovering to normal on the 14th day. After UVB modeling, CCL2 and Kir 4.1 were significantly up-regulated(P<0.01) and then gradually decreased. After blocking CCL2 synthesis by Bindarit injection into DRG, Kir 4.1 was significantly down-regulated(P<0.01), while Kir 4.1 was up-regulated after CCL2 injection(P<0.05). Conclusion The production of peripheral hyeralgesia induced by UVB irradiation is related to the upreguletion of CCL2 in DRG, and the upregulation of Kir 4.1 secondary to the change of CCL2 may play a role in inhibiting the increase of neuronal excitability.

关 键 词：紫外线 背根节 痛觉敏化 炎症 

分 类 号：R594[医药卫生—内科学]