膳食结构对鞭虫感染人群肠道菌群的影响研究  

Effect of dietary structures on intestinal flora in population infected with Trichuris trichura

作　　者：张艺馨 王龙江 刘建成 刘萍萍 王用斌[1] 许艳[1] 闫歌[1] 卜秀芹[1] 张佃波[1] 李曰进 张本光[1] ZHANG Yi-xin;WANG Long-jiang;LIU Jian-cheng;LIU Ping-ping;WANG Yong-bin;XU Yan;YAN Ge;BU Xiu-qin;ZHANG Dian-bo;LI Yue-jin;ZHANG Ben-guang(Shandong Institute of Parasitic Disease Control,Shandong First Medical University(Shandong Academy of Medical Sciences),Jining 272033,Shandong Province,China;Linyi Center for Disease Control and Prevention;Disease Control and Prevention Center of Lanshan District,Rizhao City)

机构地区：[1]山东省寄生虫病防治研究所,山东第一医科大学(山东省医学科学院),山东济宁272033 [2]临沂市疾病预防控制中心 [3]日照市岚山区疾病预防控制中心

出　　处：《热带病与寄生虫学》2023年第1期35-43,56,共10页Journal of Tropical Diseases and Parasitology

基　　金：山东省医药卫生科技发展计划(202001050586);山东省自然科学基金项目(ZR2017YL005);山东第一医科大学学术提升计划(2019QL005);山东省医学科学院医药卫生科技创新工程。

摘　　要：目的 探讨不同膳食结构对鞭虫感染人群肠道菌群的影响,为研究鞭虫感染者肠道疾病与饮食关系奠定基础。方法 在山东省日照市岚山区5个乡镇选取34名鞭虫感染者,收集研究对象膳食资料,通过16S rDNA测序平台分析不同膳食结构下鞭虫感染人群肠道菌群多样性和物种组成的差异。结果 未发现膳食摄入量高低与肠道菌群α多样性或β多样性有关,但蔬菜、奶制品和肉制品三种膳食结构与鞭虫感染人群肠道菌群的丰度与组成有显著关联。不同蔬菜摄入量组在属水平上相对丰度变化差异有统计学意义的有3个菌属:每日摄入量<300 g的鞭虫感染组(PDV3组)狭义梭菌属1(Clostridium sensu stricto 1)相对丰度高于每日摄入量>500 g的鞭虫感染组(PDV1组)(t=2.211,P<0.05);PDV3组瘤胃球菌属(Ruminococcus)相对丰度低于PDV1组(t=2.246,P<0.05);PDV3组双歧杆菌属(Bifidobacterium)相对丰度低于每日摄入量300~500 g的鞭虫感染组(PDV2组)(t=2.610,P<0.05)。不同奶制品摄入量组在属水平上相对丰度变化差异有统计学意义的有3个菌属:每日摄入量300~500 g的鞭虫感染组(PDD2组)狭义梭菌属1相对丰度高于每日摄入量>500 g的鞭虫感染组(PDD1组)(t=3.025,P<0.05);每日摄入量<300 g的鞭虫感染组(PDD3组)另枝菌属(Alistipes)相对丰度低于PDD1组(t=3.234,P<0.05);PDD3组瘤胃球菌UCG-014属(Ruminococcaceae UCG-014)相对丰度低于PDD2组(t=2.255,P<0.05)。不同肉制品摄入量组在属水平上相对丰度变化差异有统计学意义的有3个菌属:每日摄入量120~200 g的鞭虫感染组(PDM2组)考拉杆菌属(Phascolarctobacterium)和瘤胃球菌属2(Ruminococcus 2)相对丰度低于PDM1组(t=2.672、2.731,P均<0.05);每日摄入量<120 g的鞭虫感染组(PDM3组)小类杆菌属(Dialister)相对丰度低于PDM2组(t=2.402,P<0.05)。结论 不同膳食结构影响鞭虫感染人群的肠道菌群组成,提示应重视日常膳食摄入对鞭虫感染人群肠道疾病的调Objective To investigate the effects of different dietary structures on the intestinal flora in population infected by Trichuris trichura(T. trichura) so as to lay a foundation for studying the relationship between intestinal diseases and diet in patients with T. trichura infection. Methods Thirty-four patients infected by T. trichura were recruited from five towns in Lanshan District, Rizhao City, Shandong Province, and their dietary information was collected and analyzed by 16S rDNA high-throughput sequencing to determine the differences in intestinal flora diversity and species composition among T. trichura infected populations with different dietary structures. Results No association was found between high dietary intake and gut flora Alpha diversity or Beta diversity. The three dietary structures of vegetables, dairy products and meat products were significantly associated with the abundance and composition of intestinal flora.There were three species with significant differences in the intake of different vegetables at the genus level. The relative abundance of Clostridium sensu stricto 1 in the PDV3 group with a daily intake of <300 g was higher than that in PDV1 group with a daily intake of >500 g, and the difference was statistically significant(t=2.211, P<0.05). The relative abundance of Ruminococcus in PDV3 group was significantly lower than that in PDV1 group(t=2.246, P<0.05), whereas the relative abundance of Bifidobacterium was lower in PDV3 group than in PDV2 group with daily intake of 300-500 g.The difference was significant(t=2.610, P<0.05). Three species were significantly different in the intake of different dairy products at the genus level. The abundance of Clostridium sensu stricto 1 in PDD2 group with daily intake of 300g-500 g was higher than that in PDD1 group with daily intake of >500 g(t=3.025, P<0.05), contrarily, the abundance of the Alistipes in PDD3 group with daily intake <300 g was lower than that in PDD1 group(t=3.234, P<0.05). The abundance of Ruminococcaceae UCG-014 was signifi

关 键 词：鞭虫 膳食结构 肠道菌群 相对丰度 多样性 

分 类 号：R383.14[医药卫生—医学寄生虫学]