HPLC法测定复元胶囊中五味子醇甲的含量  

Study on Quality Standard of Fuyuan Capsules

作　　者：周凤盈 王殿奎 耿春辉 孙琛 王夏青 ZHOU Feng-ying;WANG Dian-kui;GENG Chun-hui;SUN Chen;WANG Xia-qing(Hengshui City Comprehensive Inspection and Testing Center,Hebei Hengshui 053000,China)

机构地区：[1]衡水市综合检验检测中心,河北衡水053000

出　　处：《广州化工》2022年第24期93-96,共4页GuangZhou Chemical Industry

基　　金：河北省衡水市科技计划项目(No:2022014089Z)。

摘　　要：建立复元胶囊中五味子醇甲的含量测定方法。采用高效液相色谱(HPLC)法对复元胶囊中五味子主要成分五味子醇甲(C_(24)H_(32)O_(7))进行含量测定;流动相为甲醇-水(65∶35),检测波长为250 nm。五味子醇甲(C_(24)H_(32)O_(7))在4.096~40.96μg/mL范围内溶液浓度与峰面积存在良好的线性关系,定量限为0.399μg/mL,检测限为0.120μg/mL,平均回收率为94.4%,RSD为0.6%(n=6);精密度试验、重复性试验、稳定性试验均良好。该方法灵敏度高、准确可靠,可以有效地控制复元胶囊的质量。A method for the determination of schisandrol A in Fuyuan capsules was established. The content of schisandrin A(C_(24)H_(32)O_(7)), as the main component of Schisandra chinensis in Fuyuan Capsules, was determined by high performance liquid chromatography(HPLC), the mobile phase was methanol-water(65∶35), and the detection wavelength was 250 nm. Schizandrin A(C24H32O7) had a good linear relationship between the solution concentration and the peak area in the range of 4.096 μg/mL to 40.96 μg/mL, the limit of quantification was 0.399 μg/mL, the limit of detection was 0.120 μg/mL, and the average recovery was 94.4%, RSD was 0.6%(n=6), the precision test, repeatability test and stability test were all good. The method has high sensitivity, accuracy and reliability, and can effectively control the quality of Fuyuan capsules.

关 键 词：复元胶囊 质量控制 定量检验 高效液相色谱法 五味子醇甲(C_(24)H_(32)O_(7)) 

分 类 号：R917[医药卫生—药物分析学]