高效液相色谱法测定烫发类化妆品中巯基乙酸的方法学验证  

Methodology Validation for Determination of Thioglycolic Acid in Perm Cosmetics by High Performance Liquid Chromatography

作　　者：严筱楠 高霞 高娟 YAN Xiao-nan;GAO Xia;GAO Juan(Yulin Institute for Drug Control,Shaanxi Yulin 719000,China)

机构地区：[1]榆林市药品检验所,陕西榆林719000

出　　处：《广州化工》2022年第24期118-119,147,共3页GuangZhou Chemical Industry

摘　　要：按照《化妆品安全技术规范》(2015年版)中“巯基乙酸”第一法测定方法。采用C18色谱柱,以乙腈-0.01 mol/L KH_(2)PO_(4)(磷酸调节pH至2.5)(8:92)为流动相,流速为1.0 mL/min,测定波长为215 nm,柱温30℃,进样量20μL。结果巯基乙酸在0.687675~275.070μg/mL响应值与浓度呈良好线性关系,方法检出限为0.003μg,方法定量限为0.012μg,平均回收率97.3%,精密度和重现性相对标准偏差(RSD)分别为0.3%和3%。该方法灵敏度高、重现性好,结果精确,可用于烫发类化妆品中巯基乙酸的含量测定。According to the determination method for thioglycolic acid in the Cosmetics Safety Technical Specifications(2015 Edition), the content of thioglycolic acid in perm cosmetics was determined by high performance liquid chromatography. Chromatography separation was carried out on a Welch C18column, which mobile phase for isocratic elution(adjusted to pH2.5 by phosphoric acid) was volume ratio of 10:90,and the flow rate of 1.0 mL/min. The detection was carried out at 215 nm with column temperature maintained at 30 ℃. Results under those conditions showed that when the mass concentration of thioglycolic acid ranged within 0.687675~275.070 μg/mL,it indicated good linear relationship. The detection limit of the method was 0.003 μg, and the limit of quantification of the method was 0.012 μg. The average recovery rate was 97.3%. The relative standard deviation(RSD) of precision and reproducibility were 0.3% and 3%, respectively. The method is suitable for perm cosmetics, because of its’ high sensitivity, good reproducibility and high accuracy.

关 键 词：巯基乙酸 烫发类化妆品 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]