EphA2蛋白与GST-pi蛋白在结直肠癌组织中表达及其预后意义  

The expression of EphA2 and GST-pi protein and their prognostic significance for colorectal cancer

作　　者：陆军[1] 张艳皊 陈洁[1] 张岩[1] LU Jun;ZHANG Yanling;CHEN Jie;ZHANG Yan(Department of Pathology,Huaibei People′s Hospital,Huaibei,Anhui 235000,China)

机构地区：[1]淮北市人民医院病理科,安徽淮北235000

出　　处：《国际检验医学杂志》2023年第5期541-547,共7页International Journal of Laboratory Medicine

基　　金：2021年度安徽省卫生健康委科研项目(AHWJ2021b138)。

摘　　要：目的探讨促红细胞生成素肝细胞A2(EphA2)蛋白与谷胱苷肽S-转移酶Pi(GST-pi)蛋白在结直肠癌标本中的表达及其与临床病理特征、预后的关系。方法收集2014年6月至2020年2月于该院接受手术治疗的114例结直肠癌患者,应用免疫组化染色技术检测EphA2蛋白与GST-pi蛋白在癌组织及其对应癌旁组织中的表达,分析二者与结直肠癌临床病理特征及总生存率的关系。结果EphA2蛋白与GST-pi蛋白的阳性表达率分别为54.4%和62.3%,显著高于二者在癌旁组织中的表达,差异有统计学意义(P<0.05)。与EphA2蛋白阴性表达患者相比,EphA2蛋白阳性表达患者的肿瘤最大径>5 cm占比、有淋巴结转移占比、有淋巴血管侵犯占比更高;GST-pi蛋白表达与结直肠癌患者的肿瘤最大径及T分期有关(P<0.05)。Kaplan-Meier生存分析表明EphA2蛋白阳性表达与阴性表达患者5年总生存率分别为20.1%和68.8%,差异有统计学意义(χ^(2)=16.973,P<0.001);GST-pi蛋白阳性表达与阴性表达者的5年总生存率分别为28.2%和61.8%,差异有统计学意义(χ^(2)=3.974,P=0.046)。单、多变量Cox回归分析表明,EphA2蛋白阳性表达(风险比=3.442,95%CI:1.722~6.881,P<0.001)、GST-pi蛋白阳性表达(风险比=2.203,95%CI:1.088~4.460,P=0.028)、淋巴结转移(风险比=3.627,95%CI:1.699~7.742,P=0.001)及淋巴血管侵犯(风险比=2.113,95%CI:1.033~4.322,P=0.040)是结直肠癌患者预后不佳的独立预测因素。结论EphA2蛋白与GST-pi蛋白或许可作为结直肠癌患者预后评估的重要参考指标。Objective To detect the expression of erythropoietin-producing hepatocyte A2(EphA2)protein and glutathione S-transferase-pi(GST-pi)protein in colorectal cancer samples and evaluate their correlation to clinicopathological characteristics and the prognosis of colorectal cancer patients.Methods The samples were collected from 114 patients who underwent surgical resection for colorectal cancer in a hospital from June 2014 to February 2020.The expression levels of EphA2 protein and GST-pi protein in cancer tissues and matched adjacent tissues were detected by immunohistochemical staining,and their relationships to clinicopathological characteristic and overall survival rate of colorectal cancer patients was analyzed.Results The positive expression rates of EphA2 protein and GST-pi protein were 54.4%and 62.3%,respectively,which were significantly higher than those of EpHA2 protein and GST-pi protein in adjacent tissues,and the difference was statistically significant(P<0.05).Compared with patients with EphA2 protein negative expression,patients with EphA2 protein positive expression had a higher proportion of tumors with maximum diameter>5 cm,lymph node metastasis and lymph vessel invasion.The expression of GST-pi protein was correlated with the maximum tumor diameter and T stage of colorectal cancer patients(P<0.05).Kaplan-Meier survival analysis showed that the 5-year overall survival rates of patients with EphA2 protein positive expression and negative expression were 20.1%and 68.8%,respectively,with statistical significance(χ^(2)=16.973,P<0.001).The 5-year overall survival rates were 28.2%and 61.8%for those with positive and negative expression of GST-pi protein,respectively(χ^(2)=3.974,P=0.046).There was a significant survival difference between the two groups.Univariate and multivariate Cox regression analysis revealed that the positive expression of EphA2 protein(hazard ratio=3.442,95%CI:1.722-6.881,P<0.001),the positive expression of GST-pi protein(hazard ratio=2.203,95%CI:1.088-4.460,P=0.028),lymph node met

关 键 词：结直肠癌 促红细胞生成素肝细胞A2 谷胱苷肽S-转移酶Pi 临床病理特征 预后 

分 类 号：R285.5[医药卫生—中药学]