丹荔输通汤对慢性输卵管炎性阻塞模型大鼠CTGF、Smad7的影响  

Effect of Danli Shutong Decoction on CTGF and Smad7 in rats with chronic tubal inflammatory obstruction

作　　者：乔江[1] 王叶 吴肖男 孙晓峰[3] 刘祎 陈智芬 QIAO Jiang;WANG Ye;WU Xiaonan;SUN Xiaofeng;LIU Yi;CHEN Zhifen(Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha,Hunan 410007,China;First Clinical College of Chinese Medicine,Hunan University of Traditional Chinese Medicine,Changsha,Hunan 410208,China;Teaching and Research Office of Histology and Embryology,Hunan University of Traditional Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学第一附属医院妇产科,湖南长沙410007 [2]湖南中医药大学第一中医临床学院,湖南长沙410208 [3]湖南中医药大学组织胚胎学教研室,湖南长沙410208

出　　处：《国际检验医学杂志》2023年第5期611-616,共6页International Journal of Laboratory Medicine

基　　金：湖南省教育厅重点项目(18A210);湖南省自然科学基金(2021JJ70032)。

摘　　要：目的探讨丹荔输通汤对慢性输卵管炎性阻塞的治疗与作用机制。方法以SD大鼠为观察对象,随机选取11只为空白组,其余以苯酚明胶化学法制作慢性输卵管炎性阻塞模型,造模成功后,将造模后大鼠随机分为模型组、丹荔输通汤高剂量灌胃+灌肠组、丹荔输通汤中剂量灌胃+灌肠组、丹荔输通汤低剂量灌胃+灌肠组、丹荔输通汤中剂量灌胃组及阿奇霉素混悬液灌胃对照组,每组11只。实验处理30 d后,处死各组大鼠,取输卵管组织,经病理检测观察输卵管组织的组织形态变化,以逆转录-实时定量聚合酶链反应、Western blot检测输卵管组织Smad7、结缔组织生长因子(CTGF)在大鼠输卵管组织的表达。结果病理检测发现造模成功,经药物治疗后,输卵管局部组织形态趋于正常,输卵管积水、积脓消失。丹荔输通汤各剂量组均能明显降低模型组大鼠输卵管组织CTGF表达,上调Smad7表达,与模型组相比,差异有统计学意义(P<0.05),且丹荔输通汤高剂量灌胃+灌肠组、丹荔输通汤中剂量灌胃+灌肠组对大鼠输卵管组织Smad7、CTGF的影响优于丹荔输通汤低剂量灌胃+灌肠组。对于丹荔输通汤中剂量的不同用药方式上:与丹荔输通汤中剂量灌胃组相比,丹荔输通汤中剂量灌胃+灌肠组大鼠输卵管组织Smad7、CTGF相对表达水平比较,差异有统计学意义(P<0.05)。结论丹荔输通汤可能通过调节输卵管组织的CTGF及Smad7表达发挥治疗作用,从而减轻局部组织炎性反应,改善局部组织纤维化,从而恢复输卵管正常组织形态与生理功能;不同剂量丹荔输通汤对模型大鼠输卵管组织CTGF及Smad7表达影响有差异。Objective To explore the treatment and mechanism of Danli Shutong Decoction on chronic tubal inflammatory obstruction.Methods Eleven SD rats were randomly selected as blank group,and the rest were made into chronic tubal inflammatory obstruction model by phenol gelatin chemical method.After successful modeling,the rats were randomly divided into model group,Danli Shutong Decoction high-dose gavage+enema group,Danli Shutong Decoction medium-dose gavage+enema group,Danli Shutong Decoction low-dose gavage+enema group,Danli Shutong Decoction medium-dose gavage group and azithromycin suspension gavage control group,11 in each group.After 30 days of experimental treatment,the rats in each group were sacrificed,and fallopian tube tissue was taken.The morphological changes of tubal tissues were observed by pathological examination.The expressions of Smad7 and connective tissue growth factor(CTGF)in tubal tissues of rats were detected by reverse transcription-real-time quantitative polymerase chain reaction and Western blot.Results Pathological examination found that the model was successfully established.After drug treatment,the local tissue morphology of the fallopian tube became normal,and the hydrosalpinx and empyema disappeared.Danli Shutong Decoction could significantly reduce the expression of CTGF and up-regulate the expression of Smad7 in tubal tissue of rats in model group,and the difference was statistically significant compared with model group(P<0.05).Moreover,the effects of Danli Shutong Decoction high-dose gavage+enema group and Danli Shutong Decoction medium-dose gavage+enema group on Smad7 and CTGF in tubal tissue of rats were better than those of Danli Shutong Decoction low-dose gavage+enema group.In terms of different medication methods of Danli Shutong Decoction medium-dose,compared with Danli Shutong Decoction medium-dose gavage group,the relative expression levels of Smad7 and CTGF in tubal tissue of rats in Danli Shutong Decoction medium-dose gavage+enema group had statistical significance(P<0.05).C

关 键 词：慢性输卵管炎性阻塞 丹荔输通汤 结缔组织生长因子 SMAD7 

分 类 号：R285.5[医药卫生—中药学]