PLT-F通道在异常血小板计数检测中的应用进展  

Advances of PLT-F channel in the detection of abnormal platelet count

作　　者：史东沙(综述) 胡志东(审校)[1] SHI Dongsha;HU Zhidong(Department of Clinical Laboratory,General Hospital of Tianjin Medical University,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院医学检验科,天津300052

出　　处：《国际检验医学杂志》2023年第5期628-631,640,共5页International Journal of Laboratory Medicine

摘　　要：准确的血小板计数对于止血异常的诊断和处理至关重要。阻抗法是目前临床实验室检测血小板计数的主流方法,该方法虽然能够正确计数正常血液标本中的血小板,但往往会对一些异常血液标本中血小板进行错误计数。血小板的数值极高或极低及各种干扰因素都会影响血小板计数的准确性。据此,Sysmex-XN系列血液分析系统的荧光血小板(PLT-F)通道采用了流式检测的原理,用荧光恶嗪与富含核酸的血小板细胞器特异性结合,专门染色血小板。该文对PLT-F通道在各种异常血小板计数检测中的性能做一综述,阐述其与传统血小板计数方法相比的优势,以及目前存在的局限性,以期最大限度地发挥PLT-F通道的临床应用价值。Platelet count is essential for the diagnosis and management of hemostasis abnormalities.The mainstream method of platelet count in clinical laboratory is impedance method(PLT-I).PLT-I can correctly count platelets in normal blood samples,however it tends to miscount platelets in some abnormal samples.Severe thrombocytopenia or thrombocytosis and various interference factors can affect the accuracy of platelet count.In order to improve it,the Sysmex-XN series automated hematology analyzers(Sysmex Corporation,Japan)have been installed with fluorescent platelet(PLT-F)channel for platelet analysis,which is based on a fluorescence flow cytometry method with uses of dye oxazine specifically staining nucleic acid-rich organelles in platelets.This article reviews the performance of PLT-F channel in the detection of abnormal samples.The advantages and limitations of PLT-F channel compared with traditional platelet counting methods are described in order to maximize its clinical application value.

关 键 词：荧光血小板通道 血小板计数 异常 

分 类 号：R446.11[医药卫生—诊断学]