亚致死浓度的双苯[口恶]唑酸对斑马鱼胚胎生长发育的影响  

Effects of isoxadifen-ethyl on the growth and development of zebrafish embryos at sublethal concentration

作　　者：郭亚楠 李天睿 杨浩娜 刘思宏 王立峰 GUO Ya-nan;LI Tian-rui;YANG Hao-na;LIU Si-hong;WANG Li-feng(Longping Branch,College of Biology,Hunan University,Changsha 410082,China;Hunan Academy of Agricultural Sciences,Hunan Rice Research Institute,Changsha 410125,China;Hunan Academy of Agricultural Sciences,Agricultural Biotechnology Research Institute,Changsha 410125,China;College of Life Science and Health,Hunan University of Science and Technology,Xiangtan 411201,Hunan,China)

机构地区：[1]湖南大学生物学院隆平分院,长沙410082 [2]湖南省农业科学院湖南省水稻研究所,长沙410125 [3]湖南省农业科学院湖南省农业生物技术研究所,长沙410125 [4]湖南科技大学生命科学与健康学院,湖南湘潭411201

出　　处：《农药》2023年第2期105-109,128,共6页Agrochemicals

基　　金：国家自然科学基金(32272564,32001923);国家重点研发计划项目(2021YFD1700101);湖南省重点研发项目(2020WK2014,2020WK2023)。

摘　　要：[目的]双苯[口恶]唑酸是一种除草剂安全剂,可缓解禾草灵对水稻和玉米的药害作用,扩大其在水田的使用,应评估双苯[口恶]唑酸对水生生物的毒性。[方法]研究双苯[口恶]唑酸对斑马鱼胚胎的急性毒性、生长发育的影响及影响生长发育的机理。[结果]双苯[口恶]唑酸对斑马鱼的LC_(50)值在48、96 h,分别为1.81、1.66 mg/L。斑马鱼胚胎暴露于0.01、0.1 mg/L的双苯[口恶]唑酸,胚胎孵化时间延长,幼鱼体内SOD(超氧化物歧化酶)、CAT(过氧化氢酶)、MDA(丙二醛)、ALDH(乙醛脱氢酶)、GSH(还原型谷胱甘肽)活性增加,gstp2、cat、bcl2表达量增加,Cu/Zn-sod、Mn-sod和bax表达量减少。[结论]双苯[口恶]唑酸对斑马鱼胚胎的毒性为中毒,胚胎可以通过体内相关基因表达量的变化改变氧化应激酶活性进行解毒。[Aims] Isoxadifen-ethyl is a herbicide safener, which is used to alleviate the harm of diclofop-methyl on rice and maize. Assessing the toxicity of isoxadifen-ethyl in aquatic organism is fundamental to expand its use in paddy fields. [Methods] Acute toxicity of isoxadifen-ethyl on zebrafish embryos and its effects on embryos growth and development were determined, and the mechanism affecting their growth and development was discussed. [Results] The results showed that the LC_(50)(48, 96 h) of isoxadifen-ethyl on zebrafish were 1.81 and 1.66 mg/L. When zebrafish embryos were exposed to 0.01 and 0.1 mg/L isoxadifen-ethyl, the hatched time was prolonged, and there was no significant change in 96 h hatching rate, body length and the number of heartbeats within 60 s. The changes of enzyme and gene expression in young fish were determined to study its mechanism. The results showed that the activities of SOD(superoxide dismutase), CAT(catalase), MDA(malondialdehyde), ALDH(acetaldehyde dehydrogenase) and GSH(reduced glutathione) increased, and so does the expression of gstp2, cat and bcl2, while the expression of Cu/Zn-sod,Mn-sod and bax decreased. [Conclusions] The toxicity of isoxadifen-ethyl to zebrafish embryos is moderate toxicity,and embryos can detoxify isoxadifen-ethyl by changing the activity of oxidative stress enzymes through changes in the expression of related genes.

关 键 词：双苯[口恶]唑酸 斑马鱼 氧化应激 基因表达 

分 类 号：TQ450.2[化学工程—农药化工]