不同移植部位对可注射组织工程化脂肪生存率的影响  

Effect of Different Transplantation Sites on Survival Rate of Injectable Tissue Engineered Fat

作　　者：吕成鹏 倪华 李龙 刘彦普 贺龙龙[1] LYU Chengpeng;NI Hua;LI long;LIU Yanpu;HE Longlong(Key laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology of Xi'an Jiaotong University,Xi'an 710004,Shaanxi,China;Tissue Engineering Center,School of Stomatology,Air Force Military Medical University,Xi’an 710032,Shaanxi,China;Department of Stomatology,Shenzhen Nanshan District Shekou People’s Hospital,Shenzhen 518066,Guangzhou,China;Department of Oral and Maxillofacial Surgery,School of Stomatology,Airforce Military Medical University,Xi’an 710032,Shaanxi,China)

机构地区：[1]西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室,陕西西安710004 [2]空军军医大学口腔医院组织工程研发中心,陕西西安710032 [3]广东省深圳市南山区蛇口人民医院口腔科,广东深圳518066 [4]空军军医大学口腔医院口腔颌面外科,陕西西安710032

出　　处：《中国美容医学》2023年第2期5-8,共4页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金(编号:81070820);陕西省科学技术厅社会发展领域项目资助课题(编号:2019SF-161)。

摘　　要：目的:研究裸鼠腹股沟及背部两个不同部位注射移植组织工程化脂肪的存活率。方法:体外分离培养的兔脂肪来源的脂肪基质干细胞(ADSCs)和富含血小板血浆(PRP)与自体兔脂肪颗粒混合注射于裸鼠皮下,按照移植部位分为背部组和腹股沟组,分别于移植后1周、2周、4周、6周,通过B超检测方法评价移植物体积变化情况。结果:脂肪移植后第1、2、4、6周,背部组脂肪移植物存活率为(84.39±7.41)%、(70.89±4.57)%、(52.49±4.42)%、(42.44±5.42)%;腹股沟组存活率为(82.00±6.93)%、(73.81±5.59)%、(61.82±5.94)%、(51.23±4.37)%;两组存活率随时间的推移逐渐降低,通过重复测量的统计分析显示两组存活率差异具有统计学意义(P<0.05),腹股沟组脂肪移植物存活率高于背部组。结论:裸鼠腹股沟的组织工程化脂肪移植存活率更高。Objective To investigate the effect of different host sites on the survival rate of injectable tissue engineered fat.Methods Adipose derived mesenchymal stem cells(ADSCs)and platelet rich plasma(PRP)were isolated and cultured in vitro and injected subcutaneously into nude mice.According to the transplantation site,they were divided into back group and groin group.At the 1st,2nd,4th,6th weeks after transplantation,the changes of graft volume were evaluated by B-ultrasonography.Results At the 1st,2nd,4th,6th weeks after transplantation,the survival rates of fat grafts in back group were(84.39±7.41)%,(70.89±4.57)%,(52.49±4.42)%,(42.44±5.42)%,and the groin group were(82.00±6.93)%,(73.81±5.59)%,(61.82±5.94)%,(51.23±4.37)%.Statistical analysis of repeated measurements showed that the survival rate of the two groups was statisticaly diferent(P<0.05),and the survival rate of fat graft in the groin group was higher than that in the back group.Conclusions Inguinal transplantation in nude mice can improve the survival rate of injectable tissue engineered fat transplantation.

关 键 词：脂肪基质干细胞 富含血小板血浆 脂肪移植 存活率 组织工程 

分 类 号：R622[医药卫生—整形外科]