不同光照强度对两份五味子资源PSII活性的影响  

Effect of different light intensity on PSII activity of two resources of Schisandra chinensis

作　　者：郭建辉 王振兴 孙丹 石广丽 张苏苏 艾军 于淼[1] 刘雨萌 吴姝逸 GUO Jian-hui;WANG Zhen-xing;SUN Dan;SHI Guang-li;ZHANG Su-su;AI Jun;YU Miao;LIU Yu-meng;WU Shu-yi(College of Horticulture,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学园艺学院,长春130118

出　　处：《西南农业学报》2022年第12期2788-2793,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2019YFC1710701);财政拔尖人才支持计划(101020143);吉林省科技发展项目(20200404017YY)。

摘　　要：【目的】探究不同光照强度对五味子PSII活性的影响。【方法】以两份五味子‘早红’与‘铁岭-0601’资源为试材,对二者叶片进行0、600、1200、1800μmol/(m^(2)·s)光照处理。【结果】强光易导致光合机构受到光损伤。强光胁迫使得五味子叶片PSII供体侧与受体侧受到伤害,电子传递受阻,产生大量活性氧(ROS),使丙二醛(MDA)积累量增加,发生膜质过氧化。强光处理下,‘早红’与‘铁岭-0601’均出现K点,二者K点相对可变荧光(V_(K))与J点相对可变荧光(V_(J))随着光照强度的增加而不断升高,1800μmol/(m^(2)·s)光强处理下V_(J)最高;各处理下‘铁岭-0601’V_(J)均高于‘早红’。与0μmol/(m^(2)·s)相比,强光处理下‘早红’与‘铁岭-0601’电子传递效率(φ_(o))、电子传递的量子产额(φ_(Eo))、单位PSII叶绿素天线可还原QA反应中心的数量(RC/ABS)、单位面积电子传递的量子产额(ET_(O)/CS_(O))、单位面积捕获的光能(TR_(O)/CS_(O))下降,用于热耗散的量子比率(φ_(Do))、单位面积吸收的光能(ABS/CS_(O))、单位面积的热耗散(DI_(O)/CS_(O))上升。随着光照强度的升高,‘早红’与‘铁岭-0601’的MDA积累量增加,且各处理下‘铁岭-0601’的MDA积累量均高于‘早红’。‘早红’与‘铁岭-0601’叶片的花色苷积累量具有显著差异,前者是后者的3.16倍。【结论】五味子不同种质资源对强光的响应具有差异,‘早红’强光胁迫下PSII电子传递体活性强,抵御强光能力更强。【Objective】The present paper aimed to explore the effect of light intensity on PSII activity of different Schisandrae chinensis.【Method】The leaves of S.chinensis resources‘Zaohong’and‘Tieling-0601’were treated with 0,600,1200 and 1800μmol/(m^(2)·s)light.【Result】Strong light damaged the photosynthetic machinery easily.High light inhibited the activity of the PSII donor and recipient sides,which hindered electron transport,accumulated a significant amount of ROS and MDA,and caused membrane peroxidation.K point appeared in both‘Zaohong’and‘Tieling-0601’under strong light,and relative variable fluorescence intensity at the K-step(V_(K))and relative variable fluorescence intensity at the J-step(V_(J))increased with the increase of light intensity,and V_(J)point was the highest under a light intensity of 1800μmol/(m^(2)·s).V_(J)of‘Tieling-0601’was higher than that of‘Zaohong’in all treatments.Compared with the control,the electron transport efficiency(φ_(O)),quantum yield of electron transport(φ_(Eo)),number of reaction centers(RC/ABS),quantum yield of electron transport per unit area(ET_(O)/CS_(O))and captured light energy per unit area(TR_(O)/CS_(O))of PSII chlorophyll antenna decreased under strong light treatment.The quantum ratio for heat dissipation(φ_(Do)),the light energy absorbed per unit area(ABS/CS_(O))and the heat dissipation per unit area(DI_(O)/CS_(O))increased.With the increase of light intensity,the accumulation of malondialdehyde(MDA)in both‘Zaohong’and‘Tieling-0601’increased,and the MDA accumulation in‘Tieling-0601’was higher than that in‘Zaohong’.There was a significant difference in anthocyanin accumulation of leaves between‘Zaohong’and‘Tieling-0601’,and‘Zaohong’was 3 times higher than‘Tieling-0601’.【Conclusion】Different germplasm resources of S.chinensis had different responses to high light,and PSII electron transporter activity was stronger under‘Zaohong’high light stress,and the ability to resist high light was st

关 键 词：五味子 强光胁迫 PSII 

分 类 号：R282.2[医药卫生—中药学]