4种狂犬病病毒ELISA抗体检测试剂盒的评价  

Evaluation on Four Kinds of ELISA Kits for Rabies Virus Antibodies

作　　者：高露 刘婷芳 蔡冬冬[1] 李盛琼 尹杰 杨天俊 张亮 黄雅琳 袁东波[1] 阳爱国[1] 侯巍[1] Gao Lu;Liu Tingfang;Cai Dongdong;Li Shengqiong;Yin Jie;Yang Tianjun;Zhang Liang;Huang Yalin;Yuan Dongbo;Yang Aiguo;Hou Wiei(Sichuan Animal Disease Prevention and Control Center,Chengdu,Sichuan 610041,China;Military Veterinary Institute,Acaolemy of Military Medicine,Changchun,Jilin 130122,China;Fushun Bureau of Agricultural and Rural Affairs,Fushun,Sichuan 643200,China)

机构地区：[1]四川省动物疫病预防控制中心,四川成都610041 [2]军事医学研究院军事兽医研究所,吉林长春130122 [3]富顺县农业农村局,四川富顺643200

出　　处：《中国动物检疫》2023年第3期105-110,共6页China Animal Health Inspection

摘　　要：为评价国产狂犬病病毒ELISA抗体检测试剂盒的性能,选择4种国产品牌ELISA抗体检测试剂盒,分别检测463份已用荧光抗体病毒中和试验(FAVN)测知狂犬病病毒抗体效价的犬血清,用Kappa检验和配对卡方检验评价ELISA试剂盒与FAVN检测结果的一致性和差异性,计算ELISA试剂盒的诊断敏感性、诊断特异性、符合率和重复性等,并比较4种试剂盒的使用范围、样品稀释倍数等参数。结果显示:经Kappa检验,4种试剂盒与FAVN一致性均为弱;经配对卡方检验,A、B试剂盒与FAVN检测结果的差异不显著(P>0.05),而C、D试剂盒差异显著(P<0.05)。诊断敏感性,D试剂盒最高,B试剂盒最低;4种试剂盒诊断特异性均较低,为29.5%~57.7%;各试剂盒符合率相当,为71.3%~72.8%。综合敏感性、特异性、重复性等考量因素,得出C试剂盒更能满足免疫后抗体检测需求,A试剂盒也较好,两者可作为候选试剂盒。结果表明,4种国产狂犬病病毒ELISA试剂盒的诊断敏感性、诊断特异性、批间稳定性等性能需进一步提高,以满足基层免疫抗体监测需求。In order to evaluate the performance of domestic enzyme linked immunosorbent assay(ELISA)kits for rabies virus antibodies,four domestic brands of the kits were selected to respectively detect 463 canine sera with rabies virus antibody titer determined by the fluorescent antibody virus neutralization test(FAVN).The consistency and difference of the results by ELISA kits and FAVN were evaluated by Kappa test and paired Chi-square test,the diagnostic sensitivity,specificity,coincidence rate and repeatability of ELISA kits were calculated,and then the parameters of the four kits,such as application range and sample dilution ratio,were compared.The results showed that,by Kappa test,weak consistency between the four kits and FAVN was observed;by paired Chi-square test,there were no significant differences(P>0.05)between the results of kits A,B and FAVN,which were different from those of kits C and D(P<0.05).For diagnostic sensitivity,kit D was the highest,while kit B was the lowest.For diagnostic specificity,the four kits were low,ranging from 29.5%to 57.7%.The coincidence rate of each kit was similar,which remained from 71.3%to 72.8%.Considering sensitivity,specificity,repeatability and other factors,it was concluded that the immune antibodies could be better detected by kit C,followed by kit A,both of which might be used as a candidate.In conclusion,the performance of the four kits,including sensitivity,specificity and inter-batch stability,should be improved for the purpose of post-immunization antibody monitoring by animal disease prevention and control institutions at the grassroots level.

关 键 词：狂犬病 ELISA试剂盒 抗体效价 评价 

分 类 号：S855.3[农业科学—临床兽医学]