国产大蒜凝集素基因的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of Lectin Gene in Chinese Garlic

作　　者：刘琴 胡曼 朱颖 陈伟达 陈禅友 LIU Qin;HU Man;ZHU Ying;CHEN Weida;CHEN Chanyou(School of Life Sciences,Hubei Province Engineering Research Center for Legume Plants,Jianghan University,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学生命科学学院,湖北省豆类(蔬菜)植物工程技术研究中心,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2023年第1期28-35,共8页Journal of Jianghan University：Natural Science Edition

基　　金：湖北省豆类(蔬菜)植物工程技术研究中心开放基金项目(201903);2021年江汉大学校级科研项目(08210131)。

摘　　要：为了获得国产大蒜新的凝集素家族基因,根据NCBI数据库中已公布的大蒜凝集素基因序列设计引物,从国产大蒜的鳞茎中提取总RNA,采用RT-PCR技术分离克隆出一个大蒜凝集素基因,命名为ASA-C。ASA-C全长546 bp,包含1个471 bp的ORF,编码156个氨基酸的多肽。利用网站和分析软件对其进行生物信息学分析。结果 表明:ASA-C与已公布的同源二聚体大蒜凝集素ASAⅡ基因具有较高同源性;推测该基因编码多肽的N端有一典型跨膜结构域的信号肽,多肽序列上有3个甘露糖特异性结合凝集素共有基序(Q-D-N-V-Y)。多重序列比对以及系统进化树的结果 表明大蒜凝集素的保守性很强,这为进一步开发利用大蒜凝集素蛋白的功能奠定了基础。We obtained one novel garlic lectin gene with designed primers according to the sequence of Allium sativum agglutinin published in NCBI. The Chinese garlic bulb lectin gene,ASA-C,was cloned from RNAs by RT-PCR experiment. The entire length of the ASA-C was 546 bp containing a 471 bp ORF,which encoded 156 amino acids. The bioinformatics analysis of ASA-C was conducted by the website and analysis software. The results showed that the ASA-C was highly homology with the published garlic lectin ASAⅡ. It was speculated that there was a typical trans-membrane,one peptide biomarker,at the N-terminal domain. The sequence analysis showed three conserved motifs(Q-DN-V-Y) for mannose-binding. The multiple sequence alignment and evolutionary analysis showed that the garlic lectin protein was highly conserved,which laid the fundation for the further development and utilization of the fundation of garlic lectin protein.

关 键 词：大蒜 凝集素基因 克隆 生物信息学分析 

分 类 号：S633.4[农业科学—蔬菜学] Q943.2[农业科学—园艺学]