荧光碳点对生物体液中姜黄素的检测及细胞成像  

Detection of Curcumin in Biological Fluids and Cell Imaging by Fluorescent Carbon Dots

作　　者：李波[1] 郑玲玲 谭杰[3] 马少芳 王浩江[1] 卞伟[1] LI Bo;ZHENG Lingling;TAN Jie;MA Shaofang;WANG Haojiang;BIAN Wei(Department of Chemistry,Basic Medical College,Shanxi Medical University,Taiyuan 030001;School of Pharmacy,Shanxi Medical University,Taiyuan 030001;Department of Traditional Chinese Medicine,the First Hospital of Shanxi Medical University,Taiyuan 030001)

机构地区：[1]山西医科大学基础医学院化学教研室,山西太原030001 [2]山西医科大学药学院,山西太原030001 [3]山西医科大学第一医院中医科,山西太原030001

出　　处：《分析科学学报》2023年第1期8-14,共7页Journal of Analytical Science

基　　金：山西省自然科学基金(No.201901D111210);晋中市科技重点研发计划(社会发展)(No.Y213003)。

摘　　要：本文以廉价易得的橘皮和柠檬酸为原料,通过一步水热法合成荧光碳点(CDs)。对CDs的形貌、结构、元素组成和光学性能等进行了表征,考察了离子强度、光稳定性和储存时间对CDs的影响。所合成CDs为粒径约为4 nm的类球形结构,具有较好的稳定性。通过对CDs与姜黄素(CM)检测体系的选择性实验,发现常见的离子及氨基酸对CM的检测几乎无影响。基于CM对CDs选择性荧光猝灭的现象,构建了检测姜黄素的荧光探针。在最佳实验条件下,该探针对CM检测的线性范围为5~40μmol/L,检出限为9.7 nmol/L。探讨了CDs和CM作用机理,发现其主要是静态猝灭。该CDs应用于健康人体体液(血液、尿液)中微量CM的测定,其加标回收率为104.0%~115.0%。橘皮合成碳点可用于A549细胞进行成像,并表现出低细胞毒性。实验结果表明,该CDs在CM的分析检测以及细胞成像中具有较好的应用价值。In this paper,green fluorescent carbon dots(CDs)was synthesized by a one-step hydrothermal method with cheap and readily available orange peel and citric acid.Subsequently,the morphology,structure,elemental composition and optical properties of CDs were characterized.Then,the effects of ionic strength,optical stability and storage time on CDs were furtherly investigated.The experimental results showed that the synthesized CDs had a good stability and spherical structure with the average diameter of 4 nm.The selectivity and interference experiments of CDs and curcumin(CM)detection system showed that common ions and amino acids had little impact on the detection of CM.Based on the selective fluorescence quenching of CDs by CM,a fluorescent probe for the detection of curcumin was constructed.Under the optimal experimental conditions,the linear range of CM detection was 5.0-40.0μmol/L,and the detection limit was 9.7 nmol/L.The mechanism of CDs and CM was mainly static quenching.The CDs were applied to the determination of trace CM in healthy human body fluids(blood,urine),and the spiked recoveries were 104.0%-115.0%.In addition,the CDs were imaged on A549 cells and showed low cytotoxicity.Therefore,the CDs have a good application value in the field of analysis,detection and cell imaging.

关 键 词：碳点 姜黄素 细胞成像 

分 类 号：O657.39[理学—分析化学]