基于双核酸适配体的夹心试纸条法在多菌灵检测中的应用研究  

Application of Double-Nucleic Acid Aptamer Sandwich Strip Method in Carbendazim Detection

作　　者：高加乐 刘诺亚 宋玉竹[1] 张金阳[1] 韩芹芹[1] GAO Jiale;LIU Nuoya;SONG Yuzhu;ZHANG Jinyang;HAN Qinqin(Facultu of science and Technology,Kunming University of Science and Technology,Kunming 650500)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650000

出　　处：《分析科学学报》2023年第1期81-86,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.32160601);云南省基础研究项目(No.202001AT070033)。

摘　　要：建立了一种胶体金标记核酸适配体的试纸条用于多菌灵的快速检测。将核酸适配体CZ13与AuNPs结合作为信号探针,生物素标记的核酸适配体CZ12固定在链霉亲和素包被膜上作为捕获探针。优化了影响灵敏度的实验条件,如信号探针制备中使用的NaCl和适配体的浓度,检测线上链霉亲和素与生物素标记的核酸适配体的摩尔比等。在最佳实验条件下,多菌灵检测范围为0.5~40μg/mL,检测限为0.45μg/mL。该方法可用于实际样品检测,检测时间为15 min,与果蔬中常见农残不存在交叉反应,方法准确、可靠、使用简便,适合样品的现场快速检测。Carbendazim is widely used in agricultural production to control fruit and vegetable diseases,but it also destroys human endocrine system.Carbendazim residues in food have become a growing concern.In this study,an AuNPs-labeled aptamer test strip was established for the rapid detection of carbendazim.The aptamer CZ13 combined with AuNPs as a signal probe,and the biotin labeled aptamer CZ12 was immobilized on the streptavidin membrane as a capture probe.The experimental conditions affecting the sensitivity were optimized,including the concentration of NaCl and aptamer used in signal probe preparation,and the mole ratio of streptavidin and biotin-labeled aptamer on the test line.Under the optimal experimental conditions,the detection range was 0.5-40μg/mL,and the detection limit was 0.45μg/mL.The method can be used for the actual sample detection,detection time is 15 min,and there is no cross reaction with common agricultural residues in fruits and vegetables.The method is accurate,reliable,simple to use,and suitable for the rapid detection of samples in the field.

关 键 词：多菌灵 核酸适配体 试纸条 胶体金 快速检测 

分 类 号：O657.3[理学—分析化学]