利用SSR分子标记鉴定“中丝2号”丝瓜杂交种纯度  

Seed Purity Test for Luffa Hybrid’Zhongsi No.2’by SSR Molecular Markers

作　　者：宋波 杨晓 刘高峰 郑胤建 卞中华 何莉梅 练华山[2] 荆赞革 李清明 杨其长 SONG Bo;YANG Xiao;LIU Gaofeng;ZHENG Yinjian;BIAN Zhonghua;HE Limei;LIAN Huashan;JING Zange;LI Qingming;YANG Qichang(Institute of Urban Agriculture,Chinese Academy of Agricultural Sciences,Chengdu 611130,China;School of Agriculture and Horticulture,Chengdu Agricultural Science and Technology Vocational College,Chengdu 611130,China;College of Agriculture and Life Sciences,Kunming University,Kunming 650217,China)

机构地区：[1]中国农业科学院都市农业研究所,四川成都611130 [2]成都农业科技职业学院农业园艺学院,四川成都611130 [3]昆明学院农学与生命科学学院,云南昆明650217

出　　处：《蔬菜》2023年第3期10-14,共5页Vegetables

基　　金：中国农业科学院科技创新工程(34-IUA-03);四川省科技厅应用基础研究项目(2021YJ0307)。

摘　　要：为了建立一套丝瓜种子纯度的快速鉴定方法,采用SSR分子标记对丝瓜杂交一代品种“中丝2号”的纯度进行鉴定,利用24对SSR引物对“中丝2号”丝瓜及其亲本的DNA进行扩增。结果表明:从24对引物中筛选出1对特异性引物ZS-SSR24,能够分别在“中丝2号”母本和父本中扩增出特异的2条多态性条带,大小分别为220 bp和250 bp,F_(1)继承双亲的特异性条带,利用此标记能够鉴定出混在F_(1)中的父本或母本。经田间鉴定,供试丝瓜种子纯度为91.3%,这与分子标记的结果完全吻合,故该标记能够用于“中丝2号”丝瓜品种的纯度鉴定。In order to establish a rapid identification method for seed purity of luffa,SSR molecular markers were used to identify the purity of luffa hybrid’Zhongsi No.2’,and 24 pairs of SSR primers were used to amplify the DNA of luffa’Zhongsi No.2’and its parents.The results showed that a pair of specific primers ZS-SSR24 was selected from 24 pairs of primers,and two specific polymorphic bands were amplified from the female and male parents of’Zhongsi No.2’,the size of which were 220 bp and 250 bp,respectively.F_(1)inherited the specific bands of parents,and the male or female parents mixed in F_(1)could be identified by using this marker.Field identification showed that the seed purity of the tested luffa was 91.3%,which was completely consistent with the molecular marker results.Therefore,the marker could be used for the purity identification of’Zhongsi No.2’luffa cultivar.

关 键 词：丝瓜 中丝2号 纯度 SSR 

分 类 号：S642.4[农业科学—蔬菜学]