山青木水煎剂对急性酒精肝损伤保护的研究  

Protective Effect of Meliosma kirkii Hemsl.et Wils. Decoction on Acute Alcoholic Liver Injury

作　　者：蒋甜甜 豆斌斌 邢慧欣 穆子诚 方金峰 张影 王晓岩 JIANG Tiantian;DOU Binbin;XING Huixin;MU Zicheng;FANG Jinfeng;ZHANG Ying;WANG Xiaoyan(College of Chinese Medicine,Jilin Agricultural Science and Technology College,Jilin 132101,China)

机构地区：[1]吉林农业科技学院中药学院,吉林吉林132101

出　　处：《黑龙江农业科学》2023年第3期79-83,共5页Heilongjiang Agricultural Sciences

基　　金：吉林省高等学校大学生创新创业训练计划项目(2020061)。

摘　　要：为了促进山青木在预防和治疗酒精肝方面的开发利用。以民间解酒方山青木水煎剂为研究对象,通过急性毒性试验,判断其安全性,观察酒后小鼠的行为指标,测定血乙醇含量变化,建立小鼠醉酒模型,检测丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)以及超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、丙二醇(MDA)的水平,初步评价小鼠肝脏受损情况,并记录身体质量。结果表明,山青木水煎液剂量为30~35 mL·kg^(-1),各组小鼠均未出现死亡情况,说明山青木水煎剂在试验剂量下具有安全性。小鼠最佳致醉剂量为16 mL·kg^(-1)的56°白酒。山青木不同剂量组与模型对照组相比,醉酒时间均显著延长,高、中剂量组苏醒时间均降低。给药2 h后,山青木高、中剂量组能够显著降低血乙醇浓度,同时降低小鼠血清ALT、AST水平,且使小鼠肝匀浆的GSH和SOD活性显著提高。说明山青木水煎剂安全性较高,高、中剂量组解酒护肝效果良好。In order to promote the development and utilization of Meliosma kirkii Hemsl. et Wlils.in the prevention and treatment alcoholic liver injury. The research was carried out on the antialcoholic decoction of folk midicine. The acute toxicity test was used to determine its safety, observed the behavior of drunken mice, measured the changes of blood alcohol content, established the model of drunken mice, and detected the levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), superoxide dismutase(SOD), glutathione(GSH) and propylene glycol(MDA) to evaluate liver damage, the body weight was also recorded. The results showed that there was no death occurred in each group with 30-35 mL·kg^(-1)dosage of the decoction of Meliosma kirkii, and indicating that the water decoction of Meliosma kirkii was safe at the experimental dose. The optimal intoxicating dose of 56° Baijiu for mice was 16 mL·kg^(-1). Compared with the model control group, the time of intoxication in different dosage groups of Meliosma kirkii was significantly prolonged, and the recovery time of high-dose and medium-dose groups decreased. Two hours after administration, the serum ethanol concentration and serum ALT and AST levels of mice were significantly decreased, while the activities of GSH and SOD in the liver homogenate of mice were significantly increased in in the high-dose and medium-dose groups. These results indicated that the decoction was safety, and the high and medium dose groups had a good effect on relieving alcohol and protecting liver.

关 键 词：山青木 酒精性肝病 解酒护肝 

分 类 号：R285.5[医药卫生—中药学]