检测汞离子的比色-荧光双通道探针的设计合成及应用  

Synthesis and Application of Colorimetric and Fluorescent Dual Mode Probe for Detection of Mercury Ion

作　　者：李淑雅 魏超[1] 赵晗 王美 LI Shu-Ya;WEI Chao;ZHAO Han;WANG Mei(Key Laboratory of Chemical Biology of Hebei Province,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis(Ministry of Education),College of Chemistry and Materials Science,Hebei University,Baoding 071002,China)

机构地区：[1]河北大学化学与材料科学学院,药物化学与分子诊断教育部重点实验室,河北省化学生物学重点实验室,保定071002

出　　处：《分析化学》2023年第2期204-210,I0005-I0008,共11页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21906038);河北省自然科学基金项目(No.B2022201016);河北省高等学校科学技术研究项目(No.QN2021019);河北大学多学科交叉项目(No.DXK201907)资助。

摘　　要：以7-羟基吩恶嗪酮(试卤灵)为荧光团、硫代甲酸苯酯为汞离子(Hg2+)识别基团,经一步简单有机合成反应,制备了比色-荧光双通道探针NMP。采用核磁共振氢谱(1H NMR)、核磁共振碳谱(13C NMR)和高分辨率质谱(HRMS)表征了探针结构,利用吸收光谱和荧光发射光谱测试了探针对Hg2+的选择性和灵敏度。光谱测试结果表明,探针对Hg2+具有较好的选择性和检测灵敏度,检出限(3σ)为17 nmol/L。反应溶液由淡黄色变为紫红色,可对溶液中Hg2+进行比色检测,并可用于实际水样中Hg2+的检测。采用CCK-8法测试了探针的细胞毒性,结果表明,在探针浓度低于10μmol/L时,细胞存活率高于90%。共聚焦荧光显微成像结果表明,探针具有较好的细胞通透性,可对细胞内Hg2+进行荧光成像。To detect the level of mercury ions(Hg2+)in the environment and organism,a colorimetric and fluorescent dual mode probe employing resorufin as fluorophore and benzene thiocarate as reactive group was prepared through one-step organic synthesis.The exact structure of the probe was confirmed by proton nuclear magnetic resonance(~1H NMR),carbon nuclear magnetic resonance(13C NMR)and high resolution mass spectrometry(HRMS).The probe displayed high selectivity and sensitivity to Hg2+in PBS buffer,and the detection limit was estimated to be 17 nmol/L(3σ).The color of the solution changed from light yellow to aubergine.The probe could quantitatively detect Hg2+in actual water samples.The cytotoxicity of the probe was verified by cell counting kit-8(CCK-8)method,and it was found that the cell survival rate was higher than 90%when the concentration of the probe was below 10μmol/L.The results of cell imaging experiments showed that the probe had good cell permeability and could be used for imaging Hg2+in living cells.

关 键 词：汞离子 荧光探针 比色检测 细胞成像 

分 类 号：O657.3[理学—分析化学] Q503[理学—化学]