氮和磷共掺杂碳纳米粒子荧光猝灭选择性检测亚叶酸钙  

Selective Detection of Calcium Folinate by Fluorescence Quenching of Nitrogen and Phosphorus Codoped Carbon Nanoparticles

作　　者：李莉 李猛 杨明利 李洪雷[1] 张焕[1] 马跃 林乐儿 张明 LI Li;LI Meng;YANG Ming-Li;LI Hong-Lei;ZHANG Huan;MA Yue;LIN Le-Er;ZHANG Ming(Kangda College,Nanjing Medical University,Lianyungang 2200 China;Department of Thoracic Surgery,The Second People's Hospital of Lianyungang,Lianyungang 2200 China)

机构地区：[1]南京医科大学康达学院,连云港222000 [2]连云港市第二人民医院胸外科,连云港222000

出　　处：《分析化学》2023年第2期296-304,共9页Chinese Journal of Analytical Chemistry

基　　金：江苏省高校优秀中青年教师和校长境外研修计划基金项目;江苏高校“青蓝工程”项目(No.KD2021qljs001);连云港高新区科技计划项目(No.HZ201906);连云港市“海燕计划”项目(No.2020-QD-005);江苏省大学生创新创业训练计划项目(Nos.202113980012Y,202113980014Y);南京医科大学康达学院科研发展基金项目(Nos.KD2020KYJJZD074,KD2021KYJJZD014,KD2021KYRC003,KD2021KYRC015)资助。

摘　　要：以磷酸铵((NH4)3PO4)和抗坏血酸钠为原料,通过一步水热法合成了氮和磷共掺杂碳纳米粒子(N/P-CNPs),量子产率为19%。基于亚叶酸钙(CF)对N/P-CNPs的快速、高选择性猝灭,建立了测定CF的新方法。在最优实验条件下,在0.2~108.5μmol/L范围内,CF的浓度与N/P-CNPs的荧光猝灭程度((F_(0)–F)/F_(0),F_(0)为N/P-CNPs的初始荧光强度,F为加入CF后的荧光强度)呈良好的线性关系,检出限为0.05μmol/L。经实验验证,CF对N/P-CNPs的荧光猝灭机理为内滤效应和静态猝灭效应。将本方法用于实际样品中CF含量的检测,所得结果与高效液相色谱法测定结果基本一致,表明本方法具有良好的实用性,为检测CF提供了一种新策略。Nitrogen and phosphorus codoped carbon nanoparticles(N/P-CNPs)were synthesized by one-step hydrothermal method using ammonium phosphate and sodium ascorbate as raw materials,with a quantum yield of 19%.Based on the rapid and highly selective quenching of N/P-CNPs by calcium folinate(CF),a new method for quantitative determination of CF in CF injection samples was established.Under the optimal experimental conditions,the concentration of CF in the range of 0.2–108.5μmol/L and the degree of fluorescence quenching of N/P-CNPs((F_(0)–F)/F_(0),F_(0)is the initial fluorescence intensity,Fis the fluorescence intensity after adding CF)showed a good linear relationship.The detection limit was 0.05μmol/L.Besides,it was verified that the fluorescence quenching mechanism of CF on N/P-CNPs was inner filter effect and static quenching effect.The established method was used to detect the content of CF in actual samples,and the obtained results were basically consistent with the results of high performance liquid chromatography,indicating that the method had good practicability and provided a new strategy for the detection of CF.

关 键 词：氮和磷共掺杂 碳纳米粒子 荧光 亚叶酸钙 内滤效应 静态猝灭 

分 类 号：O657.3[理学—分析化学] R313[理学—化学]