mmu-FUT8-0001在糖尿病小鼠模型中视网膜组织的表达变化  

The expression change of mmu-FUT8-0001 in the retina of diabetic rats

作　　者：刘畅 王硕 贾雯 张维 张蕊 朱恺 顾永昊 LIU Chang;WANG Shuo(Department of Ophthalmology,Provincial Hospital Affiliated to Anhui Medical University,230001,Anhui Province,China)

机构地区：[1]安徽医科大学附属省立医院眼科,合肥230001 [2]中国科学技术大学附属第一医院(安徽省立医院),合肥230001

出　　处：《实用防盲技术》2023年第1期8-11,F0003,共5页Journal of Practical Preventing Blind

基　　金：安徽省自然科学基金项目(1908085MH254)。

摘　　要：目的 探究在糖尿病小鼠的视网膜组织中mmu-FUT8-0001的表达情况,为糖尿病性视网膜病变的诊断以及治疗提供新的方向,方法 将雄性SD小鼠30只分为两组,实验组的SD小鼠采用65 mg/kg链脲佐菌素(STZ,Sigma,USA)腹腔注射诱导。建立糖尿病视网膜病变模型。同时,对照组小鼠腹腔注射柠檬酸盐缓冲液。用荧光定量PCR(RT-QPCR)检测糖尿病小鼠与对照组的小鼠中视网膜组织的mmu-FUT8-0001的差异表达情况,结果 RT-QPCR结果显示,与对照组相比较,mmu-FUT8-0001在糖尿病小鼠模型中的视网膜组织中表达显著升高,其相对表达量为1.444±0.111 (P=0.02)。结论 mmu-FUT8-0001在糖尿病小鼠模型中的视网膜组织中表达显著升高,mmu-FUT8-0001未来可能成为糖尿病视网膜病变诊断治疗的的新型的分子之一。Objective To explore the expression of mmu-FUT8-0001 in the retina of diabetic rats and to provide a new direction for the diagnosis and treatment of diabetic retinopathy.Methods 30 male SD rats were divided into two groups.SD rats in the experimental group were induced by intraperitoneal injection of 65mg/kg streptozotoc in(STZ,Sigma,USA).The model of diabetic retinopathy was established.At the same time,the rats in the control group were intraperitoneally injected with citrate buffer.Fluorescence quantitative PCR(RT-QPCR) was used to detect the differential expression of mmu-FUT8-0001 in the retina of diabetic rats and control rats.Results The results of RT-QPCR showed that the expression of mmu-FUT8-0001 in the retina of diabetic rats was significantly higher than that of the control group,and its relative expression was 1.444±0.111.Conclusion The expression of mmu-FUT8-0001 is significantly increased in the retina of diabetic rats,and mmu-FUT8-0001 may become one of the new molecules in the diagnosis and treatment of diabetic retinopathy in the future.

关 键 词：mmu-FUT8-0001 hsa_circ_0003028 糖尿病性视网膜病变 糖尿病小鼠 RT-QPCR 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]