林麝肺成纤维细胞线粒体全基因组结构特征及遗传变异分析  

Structural Characteristics and Genetic Variation of Lung Fibroblasts in Moschus berezovskii

作　　者：唐可欣 伍茜 付文龙[2] 程建国[2] 吴杰[2] 周磊[2] 王翔 赵位 任梓薇 李依濛 刘洁 罗燕[1] TANG Kexin;WU Xi;FU Wenlong;CHENG Jianguo;WU Jie;ZHOU Lei;WANG Xiang;ZHAO Wei;REN Ziwei;LI Yimeng;LIU Jie;LUO Yan(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130.China;Sichuan Institute of Musk Deer Breeding,Dujiangyan Sichuan 611800,China)

机构地区：[1]四川农业大学动物医学院,成都611130 [2]四川养麝研究所,四川都江堰611800

出　　处：《西北农业学报》2023年第4期534-541,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：四川省科技厅四川省科研院所科技成果转化资金(2022JDZH0026)。

摘　　要：林麝(Moschus berezovskii)是一种具有较高经济价值和药用价值的保护野生动物。为深入研究林麝线粒体基因组的结构特征及遗传变异,基于IlluminaNovaSeq平台对林麝肺成纤维细胞FMD-C1线粒体进行全基因组测序,并进行特征注释、结构预测、序列分析和系统发育重建。结果显示,FMD-C1线粒体基因组序列全长为16351bp,共有13个蛋白编码基因(protein coding gene,PCG),22个tRNA,2个rRNA和两段非编码区,具有明显的AT偏好性。将序列上传至GenBank,登录号为MW879208。与NCBI上林麝的其余序列比对,FMD-C1的4个PCGs中检测出5个单氨基酸突变和3个插入氨基酸;D-loop区检测出19个差异碱基和2个碱基缺失。系统进化分析发现与林麝亲缘关系最近的是安徽麝。研究结果可为林麝的选育和遗传多样性保护提供参考依据,也为进一步探讨林麝肺部疾病线粒体水平的致病机制奠定基础。Moschus berezovskii (FMD) is a kind of nationally protected species with high value. In order to further study the structural characteristics and genetic variation of mitochondrial genome of FMD, the whole-genome of FMD-C1 mitochondria from FMD fibroblasts was sequenced based on Illu-mina novaseq platform. Then its features were annotated. Structure prediction,sequence analysis and systematic development reconstruction were carried out. The results showed the length of whole-genome sequence was 16 351 bp. With obvious AT preference, there were 13 protein coding genes (PCGs) ,22 tRNAs,2 rRNAs and two non-coding regions. The sequence was submitted to GenBank and the accession number MW879208 was obtained. Compared with other FMD mitochondrial whole-genome sequences of NCBI,5 single amino acid mutations and 3 inserted amino acids were detected in 4 PCGs of FMD-C1;19 differential bases and 2 bases deletion were detected in D-loop region. Phylogenetic analysis showed that moschus anhuiensis was the closest relative to FMD. The results provided a reference for the breeding and genetic diversity protection of FMD,and also laid a foundation for further exploring the pathogenic mechanism of mitochondrial level of lung diseases in FMD.

关 键 词：林麝 线粒体基因组 蛋白编码基因 非编码区 系统进化 

分 类 号：S865.41[农业科学—野生动物驯养]