外源24-表油菜素内酯对花后苹果短枝叶片糖代谢和成花基因表达的影响  

Effect of Exogenous 24-Epicastasterone on Glucose Metabolism and Floral Gene Expression in Apple Spur Leaves after Flowering

作　　者：杨天一 马利萍 李凯 张满让[1] YANG Tianyi;MA Liping;LI Kai;ZHANG Manrang(College of Horticulture,Northwest A&F University,Yangling Shaanxi 712100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2023年第4期577-584,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家现代农业产业技术体系(CARS-27)。

摘　　要：以24-表油菜素内酯为外源处理剂,于盛花期后35 d和39 d对'长富2号'苹果树进行0.2 mg/L、0.4 mg/L和1.0 mg/L不同浓度2次叠加处理.为探究外源油菜素内酯处理对苹果花后当年生短枝叶片可溶性糖含量影响,测定分析当年生短枝叶片可溶性糖含量变化,并对其可溶性糖和成花相关基因进行实时荧光定量PCR分析.结果表明:不同浓度外源24-表油菜素内酯处理,各可溶性糖含量发生不同变化,浓度0.4 mg/L处理在所设浓度梯度效果较好.除花后50 d外,其他时间蔗糖含量均显著高于对照;葡萄糖和果糖含量在花后50~80 d均高于对照;经外源处理后山梨糖醇含量先增加,但在70~80 d含量降低.可溶性糖相关基因表达也发生相似变化.促花基因MdSOC1、MdFT在花后50 d和70~80 d表达量均显著高于对照.以上结果表明:适宜浓度的外源油菜素内酯处理可增加当年生短枝叶片可溶性糖含量,累积源叶中碳水化合物,有利于营养物质运输,同时促花基因累积表达.In order to explore the effect of exogenous brassinosteroids on the soluble sugar content in the annual spur leaves,24-Epicastasterone was used as exogenous treatment agent,'Nagafu 2’apple trees were treated with 24-Epi at 35 and 39 days with two overlapping concentrations of 0.2 mg/L.0.4 mg/L and 1.0 mg/L after full bloom.The changes of soluble sugar content in the annual spur leaves were measured,and the quantitative real-time PCR analysis of soluble sugar and flowers related genes was conducted.The results showed that different concentrations of exogenous 24-Epicastaster-one had different changes in soluble sugar content,and the concentration of 0.4 mg/L treatment had better effect in the set concentration gradient.The content of sucrose was significantly higher than the.control at other times except for 50 days after full bloom;the content of glucose and fructose was higher than that of the control from 50-80 days after full bloom.The content of sorbitol increased af-ter exogenous treatment,but decreased at 70-80 days.Similar changes occurred in soluble sugar-re-lated gene expression.The expression of the flower promoting genes MdSOC1 and MdFT were signifi-cantly higher than those of the control at 50 days and 70-80 days after full bloom.The above results indicated that exogenous oleuropein lactone treatment at appropriate concentrations could increase the soluble sugar content of annual spur leaves,accumulate carbohydrates in the leaves,facilitate nutrient transport,and the accumulate the expression of flower-promoting genes.

关 键 词：苹果 油菜素内酯 可溶性糖 成花 基因表达 

分 类 号：S661.1[农业科学—果树学]